Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/23

From 2010.igem.org

(Difference between revisions)
(New page: ===1:DNA Digestion=== <Member><br /> Hitomi <Sample, Materials><br /> ・PCR productions *1 *2 *4 *5 <Protocol><br /> See [https://2010.igem.org/Team:Tokyo_Metropolitan/Proj...)
(2:PCR)
Line 18: Line 18:
Hitomi
Hitomi
-
<Sample><br />(temperature in annealing)
+
<Sample>(temperature in annealing)<br />
*7(67.5)
*7(67.5)
*9(57,61,69)
*9(57,61,69)
Line 24: Line 24:
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
-
 
-
 
===3:DNA extraction===
===3:DNA extraction===

Revision as of 08:12, 28 September 2010

Contents

1:DNA Digestion

<Member>
Hitomi

<Sample, Materials>
・PCR productions

  • 1
  • 2
  • 4
  • 5

<Protocol>
See Prptpcpl 9


2:PCR

<Member>
Hitomi

<Sample>(temperature in annealing)

  • 7(67.5)
  • 9(57,61,69)

<Protocol>
See Protocol 1

3:DNA extraction

<Member>
Hitomi

<Sample>
PCR products(9/23)

<Protocol>
See Protocol 4


4:Electrophoresis

<Member>
Hitomi

<Sample>
Digestion products.

  • 1
  • 2
  • 3
  • 4
  • 5

<Protocol>
See Protocol 8

<Result>
[[Image:]]

5:Electrophoresis

<Member>
Hitomi

<Sample>
PCR products (9/23)

<Protocol>
See Protocol 8

<Result>
[[Image:]]


6:DNA extraction from gels

<Member>
Mariko,Hitomi

<Sample>
Digestion products (9/23) (After electrophoresis)

<Protocol>
See Protocol 4


7:DNA Digestion

<Member>
Hitomi

<Sample, Materials>

  • 2 (9/23)
  • 4 (9/23)

<Protocol>
See Prptpcpl 9