Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/11

From 2010.igem.org

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[[Image:Matsuura_2010-08-06_20hr_02min_(20).JPG‎]]
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===4:DNA extraction===
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===4:Electrophoresis===
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<Member><br />
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mio
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<Sample><br />
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Digestion products (9/11)
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*1
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*2
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*4
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*5
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<Protocol><br />
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
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<Result><br />
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===5:DNA extraction===
<Member><br />
<Member><br />
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<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
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===5:Digestion(2)===
===5:Digestion(2)===

Revision as of 07:09, 28 September 2010

Contents

1:PCR

<Member>
mio,easily


<Sample>

  • 2


<Protocol>
See Protocol 2

(temperature in annealing)

  • 2:70.0℃


2:Digestion(1)

<Member>
Mariko,mizuki


<Sample>

  • 1H:Avr2,FD Buffer
  • 2(9/6):Nhe1、M Buffer
  • 4(8/30):Avr2,FD Buffer
  • 5(8/30):Spe1、M Buffer


<Protocol>
See Prptpcpl 9


3:Electrophoresis

<Member>
Mariko,mio


<Sample>

  • PCR products(9/11)
  • Digestion products(9/11)


<Protocol>
See Protocol 8


<Result>
Matsuura 2010-08-06 20hr 02min (20).JPG

4:Electrophoresis

<Member>
mio


<Sample>
Digestion products (9/11)

  • 1
  • 2
  • 4
  • 5

<Protocol>
See Protocol 8


<Result>



5:DNA extraction

<Member>
mizuki,easily


<Sample>
PCR products(9/11)


<Protocol>
See Protocol 4

5:Digestion(2)

<Member>
easily


<Sample>

  • 1(9/11)


<Protocol>
See Prptpcpl 9


6:Electrophoresis

<Member>
Mariko,mizuki


<Sample>
Digestion products

  • 1(9/11)
  • 4L(8/26)
  • 8H


<Protocol>
See Protocol 8


<Result>
Matsuura 2010-08-06 20hr 02min (22).JPG

8:DNA extraction

<Member>
mio,mizuki


<Sample>

  • 4(9/11)
  • 5(9/11)


<Protocol>
See Protocol 4


9:Ligation

<Member>
Mariko,mio


<Sample>

  • 4(9/11)
  • 5(9/11)


<Protocol>
See Protocol 10


10:DNA extraction

<Member>
Mariko,mio,mizuki


<Sample>

  • 1(9/11)
  • 2(9/11)


<Protocol>
See Protocol 4


12:Ligation

<Member>
Mariko


<Sample>

  • 1(9/11)
  • 2(9/11)


<Protocol>
See Protocol 10