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Revision as of 03:44, 9 August 2010 (view source) Shaothing (Talk | contribs) (→Protocols) ← Older edit		Revision as of 12:33, 25 September 2010 (view source) Shaothing (Talk | contribs) (→Protocols) Newer edit →
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	# Plasmid DNA miniprep		# Plasmid DNA miniprep
	# 3A BioBrick assembly		# 3A BioBrick assembly
		+	# PCR cloning
		+	# PCR site-directed mutagenesis
		+	# Extraction of genome DNA for PCR cloning

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Revision as of 12:33, 25 September 2010

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Protocols

1. Preparation of competent cells for heat-shock transformation
2. Transformation by heat-shock
3. Plasmid DNA miniprep
4. 3A BioBrick assembly
5. PCR cloning
6. PCR site-directed mutagenesis
7. Extraction of genome DNA for PCR cloning