Team:UNIPV-Pavia/Calendar/September/settimana4

(Difference between revisions)

Revision as of 13:50, 24 September 2010

SEPTEMBER: WEEK 4

Screening PCR on 6 colonies picked from I55 was performed. The results were the follow:

I55.

Inoculum of I52, I53, I55, I56 and I57 in LB+Amp for MiniPrep of the following day

Miniprep and Nanodrop quantification for:

For I61 the DNA is already available quantified 147,1 ng/ul.

After 3 hour digestion was performed:

Gel run/cut of samples

As you can see only I52, I56, I57 and I61 are positive so gel extraction was performed on this samples:

We already had digested DNA so we could perform new ON ligations:

Tecan Test on self-inducible promoters was performed on prepared samples with a different protocol that is to bring the cultures to a known OD.

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 As you can see only I52, I56, I57 and I61 are positive so gel extraction was performed on this samples:
 * I52 = 29,4 ng/ul
 * I56 = 15,4 ng/ul
 * I57 = 24,7 ng/ul
 * I61 = 30,2 ng/ul
+We already had digested DNA so we could perform new ON ligations:
+* I62 = I59(X-P) + I39(S-P)
+* I63 = I61(E-X) + I38(E-S)
+* I64 = I52(E-X) + I58(E-S)
+* I65 = I39(S-P) + I56(X-P)
+* I66 = I57(E-X) + I60(E-S)
 ----
 <font size=4>[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test21settembre|Tecan Test]]</font> on self-inducible promoters was performed on prepared samples with a different protocol that is to bring the cultures to a known OD.