Team:UT-Tokyo/Protocols
From 2010.igem.org
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- | [[/Team:UT-Tokyo/Transformation]] | + | [[/Team:UT-Tokyo/Transformation | Transformation]] |
[[/Team:UT-Tokyo/Colony Pick Up]] | [[/Team:UT-Tokyo/Colony Pick Up]] |
Revision as of 06:38, 23 September 2010
Protocols
Notice
-You should mix reagents well
-Put in enzymes after you mixed all other reagents
-Centrifuge the tube and collect substances on the bottom before opening a reagent
/Team:UT-Tokyo/Restriction Enzyme digestion(Xba1/Pst1)—Once for all
/Team:UT-Tokyo/Restriction Enzyme digestion —One by one
/Team:UT-Tokyo/Colony PCR(Ex-Taq)
/Team:UT-Tokyo/Electrophoresis
/Team:UT-Tokyo/Gel DNA Recovery
/Team:UT-Tokyo/Producing Competent Cell
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