Revision as of 06:36, 23 September 2010

UT-Tokyo

Protocols

Notice

-You should mix reagents well

-Put in enzymes after you mixed all other reagents

-Centrifuge the tube and collect substances on the bottom before opening a reagent

@@ Line 13: / Line 13: @@
-[[Transformation]]
+[[/Team:UT-Tokyo/Transformation]]
-[[Colony Pick Up]]
+[[/Team:UT-Tokyo/Colony Pick Up]]
-[[Miniprep]]
+[[/Team:UT-Tokyo/Miniprep]]
-[[Restriction Enzyme digestion（Xba1/Pst1）—Once for all]]
+[[/Team:UT-Tokyo/Restriction Enzyme digestion（Xba1/Pst1）—Once for all]]
-[[Restriction Enzyme digestion —One by one]]
+[[/Team:UT-Tokyo/Restriction Enzyme digestion —One by one]]
-[[PCR（Pfu　Ultra）]]
+[[/Team:UT-Tokyo/PCR（Pfu　Ultra）]]
-[[PCR（Ex-Taq）]]
+[[/Team:UT-Tokyo/PCR（Ex-Taq）]]
-[[PCR（KODplus）]]
+[[/Team:UT-Tokyo/PCR（KODplus）]]
-[[Colony PCR（Ex-Taq）]]
+[[/Team:UT-Tokyo/Colony PCR（Ex-Taq）]]
-[[Electrophoresis]]
+[[/Team:UT-Tokyo/Electrophoresis]]
-[[Gel DNA Recovery]]
+[[/Team:UT-Tokyo/Gel DNA Recovery]]
-[[LB　broth]]
+[[/Team:UT-Tokyo/LB　broth]]
-[[Agarose Gel]]
+[[/Team:UT-Tokyo/Agarose Gel]]
-[[Seaquencing]]
+[[/Team:UT-Tokyo/Seaquencing]]
-[[Producing Competent Cell]]
+[[/Team:UT-Tokyo/Producing Competent Cell]]
 {{UT-Tokyo_Foot}}