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Team:UT-Tokyo/Protocols
From 2010.igem.org
(Difference between revisions)
| Line 6: | Line 6: | ||
-You should mix reagents well | -You should mix reagents well | ||
| + | |||
-Put in enzymes after you mixed all other reagents | -Put in enzymes after you mixed all other reagents | ||
| + | |||
-Centrifuge the tube and collect substances on the bottom before opening a reagent | -Centrifuge the tube and collect substances on the bottom before opening a reagent | ||
| Line 13: | Line 15: | ||
Transformation | Transformation | ||
Colony Pick Up | Colony Pick Up | ||
| + | |||
Miniprep | Miniprep | ||
| + | |||
Restriction Enzyme Transaction（Xba1/Pst1）—Once for all | Restriction Enzyme Transaction（Xba1/Pst1）—Once for all | ||
| + | |||
Restriction Enzyme Transaction —One by one | Restriction Enzyme Transaction —One by one | ||
| + | |||
PCR（Pfu Ultra） | PCR（Pfu Ultra） | ||
| + | |||
PCR（Ex-Taq） | PCR（Ex-Taq） | ||
| + | |||
PCR（KODplus） | PCR（KODplus） | ||
| + | |||
Colony PCR（Ex-Taq） | Colony PCR（Ex-Taq） | ||
| + | |||
Electrophoresis | Electrophoresis | ||
| + | |||
Gel DNA Recovery | Gel DNA Recovery | ||
| + | |||
LB broth | LB broth | ||
| + | |||
Agarose Gel | Agarose Gel | ||
| + | |||
Seaquencing | Seaquencing | ||
| + | |||
Producing Competent Cell | Producing Competent Cell | ||
Revision as of 06:01, 23 September 2010
Protocols
Notice
-You should mix reagents well
-Put in enzymes after you mixed all other reagents
-Centrifuge the tube and collect substances on the bottom before opening a reagent
Transformation Colony Pick Up
Miniprep
Restriction Enzyme Transaction（Xba1/Pst1）—Once for all
Restriction Enzyme Transaction —One by one
PCR（Pfu Ultra）
PCR（Ex-Taq）
PCR（KODplus）
Colony PCR（Ex-Taq）
Electrophoresis
Gel DNA Recovery
LB broth
Agarose Gel
Seaquencing
Producing Competent Cell
Copyright © 2010 iGEM UT-Tokyo. All rights reserved.
