"
Team:HokkaidoU Japan/Protocols
From 2010.igem.org
(Difference between revisions)
| Line 13: | Line 13: | ||
==Bacterial Transformations== | ==Bacterial Transformations== | ||
==Mini-prep (Alkaline SDS Method)== | ==Mini-prep (Alkaline SDS Method)== | ||
| - | ==PCR= | + | ==PCR== |
==Restriction Enzyme Digestions== | ==Restriction Enzyme Digestions== | ||
==DNA ligation== | ==DNA ligation== | ||
==Agarose gel electrophoresis== | ==Agarose gel electrophoresis== | ||
==Electroporation== | ==Electroporation== | ||
Revision as of 15:01, 21 September 2010
since 13 Jul 2010
since 1 Aug 2010
Protocols
- Preparation of Competent cells (E. coli DH5a)
- Bacterial Transformations
- Mini-prep (Alkaline SDS Method)
- PCR
- Restriction Enzyme Digestions
- DNA ligation
- Agarose gel electrophoresis
- Electroporation
