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Revision as of 02:50, 18 September 2010 (view source) Sho N (Talk | contribs) (→Digestion of GFP and Double Terminator) ← Older edit		Latest revision as of 17:35, 19 September 2010 (view source) Laurynas (Talk | contribs) (Replacing page with '{{Template:HokkaidoU_Japan}}<div class="linkbar"><div class="prev">September 15</div>Notebook<di...')
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-	{{Template:HokkaidoU_Japan}}	+	{{Template:HokkaidoU_Japan}}<div class="linkbar"><div class="prev">[[Team:HokkaidoU_Japan/Notebook/September15|September 15]]</div>[[Team:HokkaidoU_Japan/Notebook|Notebook]]<div class="next">[[Team:HokkaidoU_Japan/Notebook/September17|September 17]]</div></div>
-	*digestion of GFP(1-14K) and double terminator(1-23L)	+
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-	== Digestion of GFP and Double Terminator ==	+
-	Parts Information	+
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-	GFP : BBa_E0040 1-14K 720bp pSB1A3	+
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-	double terminator : BBa_B0015 1-23L 129bp pSB1AK3	+
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-	pSB1A3	+
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-	1-14K and pSB1A3 had purified with mycrocon, and 1-23L had extracted from a gel before.	+
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-	Method	+
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-	1)electrophoresed 1μl of 1-14K and 1-23L added 0.5μl 6×sample buffer to estimate concerntration of each solution.	+
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-	2)estimated concerntration from photo of electrophoresys.But I forgot to electrophorese pSB1A3 solution with the other samples,so pSB1A3 solution was done by other person.	+

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Latest revision as of 17:35, 19 September 2010