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Team:HokkaidoU Japan/Notebook/September16
From 2010.igem.org
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== Digestion of GFP and Double Terminator == | == Digestion of GFP and Double Terminator == | ||
Parts Information | Parts Information | ||
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GFP : BBa_E0040 1-14K 720bp pSB1A3 | GFP : BBa_E0040 1-14K 720bp pSB1A3 | ||
| + | |||
double terminator : BBa_B0015 1-23L 129bp pSB1AK3 | double terminator : BBa_B0015 1-23L 129bp pSB1AK3 | ||
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| + | pSB1A3 | ||
| + | |||
| + | 1-14K and pSB1A3 had purified with mycrocon, and 1-23L had extracted from a gel before. | ||
| + | |||
| + | |||
| + | Method | ||
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| + | 1)electrophoresed 1μl of 1-14K and 1-23L added 0.5μl 6×sample buffer to estimate concerntration of each solution. | ||
| + | |||
| + | 2)estimated concerntration from photo of electrophoresys.But I forgot to electrophorese pSB1A3 solution with the other samples,so pSB1A3 solution was done by other person. | ||
Revision as of 02:50, 18 September 2010
since 13 Jul 2010
since 1 Aug 2010
- digestion of GFP(1-14K) and double terminator(1-23L)
Digestion of GFP and Double Terminator
Parts Information
GFP : BBa_E0040 1-14K 720bp pSB1A3
double terminator : BBa_B0015 1-23L 129bp pSB1AK3
pSB1A3
1-14K and pSB1A3 had purified with mycrocon, and 1-23L had extracted from a gel before.
Method
1)electrophoresed 1μl of 1-14K and 1-23L added 0.5μl 6×sample buffer to estimate concerntration of each solution.
2)estimated concerntration from photo of electrophoresys.But I forgot to electrophorese pSB1A3 solution with the other samples,so pSB1A3 solution was done by other person.
