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Team:TU Delft/protocols/freezing bacterial stocks
From 2010.igem.org
(Difference between revisions)
(New page: ==Freezing of bacterial stocks== ''Materials:'' - Bacterial culture - LB medium - 80% glycerol - Centrifuge ''Protocol:'' 1. Take 5 mL bacterial cells from the Erlenmeyer of a fre...) |
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| - | + | =Freezing of bacterial stocks= | |
''Materials:'' | ''Materials:'' | ||
| - | - | + | - bacterial culture |
- LB medium | - LB medium | ||
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- 80% glycerol | - 80% glycerol | ||
| - | - | + | - centrifuge |
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1. Take 5 mL bacterial cells from the Erlenmeyer of a freshly grown culture and spin in a 15 mL tube for 10 minutes | 1. Take 5 mL bacterial cells from the Erlenmeyer of a freshly grown culture and spin in a 15 mL tube for 10 minutes | ||
| - | at 2 | + | at 2,000 rpm (Eppendorf centrifuge). |
2. Decant the supernatant without disturbing the pellet. | 2. Decant the supernatant without disturbing the pellet. | ||
3. Pipet on the pellet 0.5 mL 1% LB medium and 0.5 mL 80% glycerol and mix by vortexing and save in -80 °C freezer. | 3. Pipet on the pellet 0.5 mL 1% LB medium and 0.5 mL 80% glycerol and mix by vortexing and save in -80 °C freezer. | ||
Latest revision as of 11:05, 12 September 2010
Freezing of bacterial stocks
Materials:
- bacterial culture
- LB medium
- 80% glycerol
- centrifuge
Protocol:
1. Take 5 mL bacterial cells from the Erlenmeyer of a freshly grown culture and spin in a 15 mL tube for 10 minutes at 2,000 rpm (Eppendorf centrifuge).
2. Decant the supernatant without disturbing the pellet.
3. Pipet on the pellet 0.5 mL 1% LB medium and 0.5 mL 80% glycerol and mix by vortexing and save in -80 °C freezer.
