Team:UC Davis/protocols/gelelectrophoresis.html

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<li>Paraphrased from <a href="http://www.fermentas.com" class="help">Fermentas</a> protocol</li>
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Revision as of 21:41, 9 September 2010

Gel Electrophoresis

Materials

You will need:

  • Agarose
  • 1X TAE

Extra Notes

Be sure to aware of what concentration your gel is. The difference between a 1% and a 2% agarose gel is significant.

Procedure

 Creating a 1% gel stock
  • Dissolve 5g agarose in 500mL 1X TAE

Creating a 2% gel stock

  • Dissolve 10g agarose in 500mL 1X TAE

When gel is ready to use:

  • Have gel trays ready with the gel comb in place.
  • Microwave the gel stock. Be sure it is all melted and dissolved.
  • Pour 50mL into 1 50mL centrifuge tube. If gel is bigger, pour 100mL into 2 50mL centrifuge tubes.
  • When gel solution is lukewarm, pipette 5μL safestain into gel
  • Pour immediately and slowly into gel trays to reduce chunks and bubbles in gel.
  • Wait until gel completely solidifies.

Purpose

Although gel electrophoresis has many purposes, we use them to separate DNA fragments, specifically the insert from the plasmid.

References

We would like to take a moment to thank all of our sponsors for their very generous donations, as we could not have done this without your help!

We would also like to thank and acknowledge:
Our Advisors
Marc Facciotti
Ilias Tagkopoulos
Technical Guidance
David Larsen
Andrew Yao
Visiting iGEMer
Jia Li of Zhejiang University (TEAM ZJU-China)
cI Promoter Screen
Drew Endy - Stanford
Thomas Schneider - NIH
Want to sponsor us? Send an email to mtfacciotti@ucdavis.edu to discuss various ways you can help! :)

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