Team:Michigan/Pili Expression
From 2010.igem.org
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+ | ==9/7/2010== | ||
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+ | Started overnight cultures of 1:1000 dilutions of K12 and ΔFim::kan, both with the pBAD+FimB plasmid. | ||
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+ | ==9/8/2010== | ||
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+ | Created frozen stocks of K12 and ΔFim::kan from overnight cultures. Stored them in box 1 in the ERB -20C freezer. | ||
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+ | Ran miniprep of K12 and ΔFim::kan, using 5 ml of each culture. | ||
+ | Note: Centrifuge in ERB will only go up to 5000 rpm w/50 ml tubes, therefore we transferred the culture to several eppendorf tubes. This was probably not a good idea, because we were left with a lot of leftover supernatant and that could have diluted the buffers. In the future, we should centrifuge the culture 1 ml at a time, and add 1 ml after each cycle. | ||
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+ | Stored the product from miniprep in box 2 in the ERB -20C freezer. | ||
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Revision as of 01:50, 9 September 2010