abstract

Our goal is the creation of an "iGarden" with the biobrick standard and in order for such a concept to work, we must get our constructs into plants. We used agrobacterium-mediated transformation, first in arabidopsis, then in strawberries.

Bacterial Double Click ---- If there are two-time input for limited time, bacteria shine. == '''Overall project''' ==


{|align="justify" |We’re inspired by double-click of computer’s mouse.
It doesn’t react by first input but react by second input. So we designed bacterial DNA sequence which works like it. Furthermore, we built time-limit in genetic sequence between the first and the second input like a computer mouse. Bacteria which have the DNA sequence can distinguish between the first input and the second one. Once in a while, bacteria might receive input which wasn’t intended. But it was the first input so bacteria don’t react. Even if there was input not intended, bacteria don’t react unless the second input is entered. Moreover, when the limit-time which controlled by the genetic system is passed, the system is going to return to the initial state. So we expect this DNA sequence will work as a safety device. |align="center"| [[Image:click.jpg|200px|left|frame]] |- |} == Project Details== ===Requirement of Double Click === 1. If there is a click only one-time, nothing happens.
2. Time of clicking button doesn't matter. It just depends on the number of click. One or two.
3. After a lot of time pass from the 1st click, it will return to the initial state.
4. Click two-time in specific limited time(We call this double click.), something occur. === The Experiments === === Part 3 === == Results ==