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Team:Lethbridge/Notebook/Working Plasmids
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<tr><td>A6</td><td>sRBS</td><td></td><td></td><td>6970</td><td></td></tr> | <tr><td>A6</td><td>sRBS</td><td></td><td></td><td>6970</td><td></td></tr> | ||
<tr><td>A7</td><td>sRBS</td><td></td><td></td><td>17000</td><td></td></tr> | <tr><td>A7</td><td>sRBS</td><td></td><td></td><td>17000</td><td></td></tr> | ||
| - | <tr><td>A8</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>A8</td><td>CFP Complete</td><td></td><td></td><td>1395</td><td></td></tr> |
| - | <tr><td>A9</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>A9</td><td>pLacI</td><td></td><td></td><td>990</td><td></td></tr> |
| - | <tr><td>A10</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>A10</td><td>sRBS</td><td></td><td></td><td>1765</td><td></td></tr> |
</table><br> | </table><br> | ||
<b>Row B</b> | <b>Row B</b> | ||
<table><table border="3"> | <table><table border="3"> | ||
<tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration via<br> Spectroscopy(µg/mL)</td><td>Concentration via <br>gel quantification (µg/mL)</td></tr> | <tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration via<br> Spectroscopy(µg/mL)</td><td>Concentration via <br>gel quantification (µg/mL)</td></tr> | ||
| - | <tr><td>B1</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>B1</td><td>EYFP</td><td></td><td></td><td>1370</td><td></td></tr> |
| - | <tr><td>B2</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>B2</td><td>N-term tag</td><td></td><td></td><td>415</td><td></td></tr> |
<tr><td>B3</td><td></td><td></td><td></td><td></td><td></td></tr> | <tr><td>B3</td><td></td><td></td><td></td><td></td><td></td></tr> | ||
| - | <tr><td>B4</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>B4</td><td>pSB-NEYFP</td><td></td><td></td><td>1185</td><td></td></tr> |
| - | <tr><td>B5</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>B5</td><td>pSB-CEYFP</td><td></td><td></td><td>1055</td><td></td></tr> |
| - | <tr><td>B6</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>B6</td><td>CFP Complete</td><td></td><td></td><td>1285</td><td></td></tr> |
| - | <tr><td>B7</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>B7</td><td>sRBS-Lum-dT</td><td></td><td></td><td>4375</td><td></td></tr> |
| - | <tr><td>B8</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>B8</td><td>sRBS-Lum-dT</td><td></td><td></td><td>4780</td><td></td></tr> |
| - | <tr><td>B9</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>B9</td><td>mr. Gene mms6</td><td></td><td></td><td>2455</td><td></td></tr> |
| - | <tr><td>B10</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>B10</td><td>pBad-TetR</td><td></td><td></td><td>2915</td><td></td></tr> |
</table><br> | </table><br> | ||
<b>Row C</b> | <b>Row C</b> | ||
<table><table border="3"> | <table><table border="3"> | ||
<tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration via<br> Spectroscopy(µg/mL)</td><td>Concentration via <br>gel quantification (µg/mL)</td></tr> | <tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration via<br> Spectroscopy(µg/mL)</td><td>Concentration via <br>gel quantification (µg/mL)</td></tr> | ||
| - | <tr><td>C1</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>C1</td><td>dT</td><td></td><td></td><td>2915</td><td></td></tr> |
<tr><td>C2</td><td></td><td></td><td></td><td></td><td></td></tr> | <tr><td>C2</td><td></td><td></td><td></td><td></td><td></td></tr> | ||
<tr><td>C3</td><td></td><td></td><td></td><td></td><td></td></tr> | <tr><td>C3</td><td></td><td></td><td></td><td></td><td></td></tr> | ||
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<table><table border="3"> | <table><table border="3"> | ||
<tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration via<br> Spectroscopy(µg/mL)</td><td>Concentration via <br>gel quantification (µg/mL)</td></tr> | <tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration via<br> Spectroscopy(µg/mL)</td><td>Concentration via <br>gel quantification (µg/mL)</td></tr> | ||
| - | <tr><td>D1</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>D1</td><td>pLacI</td><td></td><td></td><td>70</td><td></td></tr> |
| - | <tr><td>D2</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>D2</td><td>sRBS-Lum-dT</td><td></td><td></td><td>95</td><td></td></tr> |
| - | <tr><td>D3</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>D3</td><td>C-term (H4)</td><td></td><td></td><td>860</td><td></td></tr> |
| - | <tr><td>D4</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>D4</td><td>C-term (I4)</td><td></td><td></td><td>1145</td><td></td></tr> |
| - | <tr><td>D5</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>D5</td><td>C-term (J4)</td><td></td><td></td><td>1805</td><td></td></tr> |
| - | <tr><td>D6</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>D6</td><td>pLacI (B1)</td><td></td><td></td><td>440</td><td></td></tr> |
| - | <tr><td>D7</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>D7</td><td>xylE (B4)</td><td></td><td></td><td>1820</td><td></td></tr> |
| - | <tr><td>D8</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>D8</td><td>xylE (C4)</td><td></td><td></td><td>420</td><td></td></tr> |
| - | <tr><td>D9</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>D9</td><td>mms6 (A6)</td><td></td><td></td><td>1530</td><td></td></tr> |
| - | <tr><td>D10</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>D10</td><td>mms6 (B6)</td><td></td><td></td><td>1440</td><td></td></tr> |
</table><br> | </table><br> | ||
<b>Row E</b> | <b>Row E</b> | ||
<table><table border="3"> | <table><table border="3"> | ||
<tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration via<br> Spectroscopy(µg/mL)</td><td>Concentration via <br>gel quantification (µg/mL)</td></tr> | <tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration via<br> Spectroscopy(µg/mL)</td><td>Concentration via <br>gel quantification (µg/mL)</td></tr> | ||
| - | <tr><td>E1</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>E1</td><td>NEYFP</td><td></td><td></td><td>235</td><td></td></tr> |
| - | <tr><td>E2</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>E2</td><td>NEYFP</td><td></td><td></td><td>2980</td><td></td></tr> |
| - | <tr><td>E3</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>E3</td><td>Fusion CEYFP</td><td></td><td></td><td>255</td><td></td></tr> |
| - | <tr><td>E4</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>E4</td><td>Fusion CEYFP</td><td></td><td></td><td>490</td><td></td></tr> |
| - | <tr><td>E5</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>E5</td><td>Fusion CEYFP</td><td></td><td></td><td>335</td><td></td></tr> |
| - | <tr><td>E6</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>E6</td><td>CEYFP</td><td></td><td></td><td>1605</td><td></td></tr> |
| - | <tr><td>E7</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>E7</td><td>CEYFP</td><td></td><td></td><td>1930</td><td></td></tr> |
| - | <tr><td>E8</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>E8</td><td>EYFP</td><td></td><td></td><td>640</td><td></td></tr> |
| - | <tr><td>E9</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>E9</td><td>EYFP</td><td></td><td></td><td>605</td><td></td></tr> |
| - | <tr><td>E10</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>E10</td><td>EYFP</td><td></td><td></td><td>620</td><td></td></tr> |
</table><br> | </table><br> | ||
<b>Row F</b> | <b>Row F</b> | ||
<table><table border="3"> | <table><table border="3"> | ||
<tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration via<br> Spectroscopy(µg/mL)</td><td>Concentration via <br>gel quantification (µg/mL)</td></tr> | <tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration via<br> Spectroscopy(µg/mL)</td><td>Concentration via <br>gel quantification (µg/mL)</td></tr> | ||
| - | <tr><td>F1</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>F1</td><td>ECFP</td><td></td><td></td><td>470</td><td></td></tr> |
| - | <tr><td>F2</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>F2</td><td>ECFP</td><td></td><td></td><td>1300</td><td></td></tr> |
| - | <tr><td>F3</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>F3</td><td>ECFP</td><td></td><td></td><td>2010</td><td></td></tr> |
| - | <tr><td>F4</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>F4</td><td>pBad-TetR</td><td></td><td></td><td>400</td><td></td></tr> |
| - | <tr><td> | + | <tr><td>F5</td><td>pBad-TetR</td><td></td><td></td><td>575</td><td></td></tr> |
<tr><td>F6</td><td></td><td></td><td></td><td></td><td></td></tr> | <tr><td>F6</td><td></td><td></td><td></td><td></td><td></td></tr> | ||
<tr><td>F7</td><td></td><td></td><td></td><td></td><td></td></tr> | <tr><td>F7</td><td></td><td></td><td></td><td></td><td></td></tr> | ||
| Line 88: | Line 88: | ||
<table><table border="3"> | <table><table border="3"> | ||
<tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration via<br> Spectroscopy(µg/mL)</td><td>Concentration via <br>gel quantification (µg/mL)</td></tr> | <tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration via<br> Spectroscopy(µg/mL)</td><td>Concentration via <br>gel quantification (µg/mL)</td></tr> | ||
| - | <tr><td>G1</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>G1</td><td>EYFP</td><td></td><td></td><td>1300</td><td></td></tr> |
| - | <tr><td>G2</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>G2</td><td>sRBS-Lum-dT(1)</td><td></td><td></td><td>335</td><td></td></tr> |
| - | <tr><td>G3</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>G3</td><td>sRBS-Lum-dT(2)</td><td></td><td></td><td>540</td><td></td></tr> |
| - | <tr><td>G4</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>G4</td><td>pSB-CEYFP</td><td></td><td></td><td>340</td><td></td></tr> |
| - | <tr><td>G5</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>G5</td><td>CFP Complete</td><td></td><td></td><td>355</td><td></td></tr> |
| - | <tr><td>G6</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>G6</td><td>pBad(1)</td><td></td><td></td><td>175</td><td></td></tr> |
| - | <tr><td>G7</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>G7</td><td>pBad(2)</td><td></td><td></td><td>315</td><td></td></tr> |
| - | <tr><td>G8</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>G8</td><td>N-term tag</td><td></td><td></td><td>270</td><td></td></tr> |
| - | <tr><td>G9</td><td></td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>G9</td><td>Lumazine</td><td></td><td></td><td>265</td><td></td></tr> |
<tr><td>G10</td><td></td><td></td><td></td><td></td><td></td></tr> | <tr><td>G10</td><td></td><td></td><td></td><td></td><td></td></tr> | ||
</table><br> | </table><br> | ||
Revision as of 01:37, 2 June 2010
Back to Notebook
Updated June 1/2010
Row A
| Cell | Common Name | Standard Registry of Parts ID | Date Entered | Concentration via Spectroscopy(µg/mL) | Concentration via gel quantification (µg/mL) |
| A1 | sRBS-Lum-dT(2) | 965 | |||
| A2 | sRBS-Lum-dT(1) | 1145 | |||
| A3 | Lumazine | 750 | |||
| A4 | pBad(1) | 1320 | |||
| A5 | pBad(2) | 2050 | |||
| A6 | sRBS | 6970 | |||
| A7 | sRBS | 17000 | |||
| A8 | CFP Complete | 1395 | |||
| A9 | pLacI | 990 | |||
| A10 | sRBS | 1765 |
Row B
| Cell | Common Name | Standard Registry of Parts ID | Date Entered | Concentration via Spectroscopy(µg/mL) | Concentration via gel quantification (µg/mL) |
| B1 | EYFP | 1370 | |||
| B2 | N-term tag | 415 | |||
| B3 | |||||
| B4 | pSB-NEYFP | 1185 | |||
| B5 | pSB-CEYFP | 1055 | |||
| B6 | CFP Complete | 1285 | |||
| B7 | sRBS-Lum-dT | 4375 | |||
| B8 | sRBS-Lum-dT | 4780 | |||
| B9 | mr. Gene mms6 | 2455 | |||
| B10 | pBad-TetR | 2915 |
Row C
| Cell | Common Name | Standard Registry of Parts ID | Date Entered | Concentration via Spectroscopy(µg/mL) | Concentration via gel quantification (µg/mL) |
| C1 | dT | 2915 | |||
| C2 | |||||
| C3 | |||||
| C4 | |||||
| C5 | |||||
| C6 | |||||
| C7 | |||||
| C8 | |||||
| C9 | |||||
| C10 |
Row D
| Cell | Common Name | Standard Registry of Parts ID | Date Entered | Concentration via Spectroscopy(µg/mL) | Concentration via gel quantification (µg/mL) |
| D1 | pLacI | 70 | |||
| D2 | sRBS-Lum-dT | 95 | |||
| D3 | C-term (H4) | 860 | |||
| D4 | C-term (I4) | 1145 | |||
| D5 | C-term (J4) | 1805 | |||
| D6 | pLacI (B1) | 440 | |||
| D7 | xylE (B4) | 1820 | |||
| D8 | xylE (C4) | 420 | |||
| D9 | mms6 (A6) | 1530 | |||
| D10 | mms6 (B6) | 1440 |
Row E
| Cell | Common Name | Standard Registry of Parts ID | Date Entered | Concentration via Spectroscopy(µg/mL) | Concentration via gel quantification (µg/mL) |
| E1 | NEYFP | 235 | |||
| E2 | NEYFP | 2980 | |||
| E3 | Fusion CEYFP | 255 | |||
| E4 | Fusion CEYFP | 490 | |||
| E5 | Fusion CEYFP | 335 | |||
| E6 | CEYFP | 1605 | |||
| E7 | CEYFP | 1930 | |||
| E8 | EYFP | 640 | |||
| E9 | EYFP | 605 | |||
| E10 | EYFP | 620 |
Row F
| Cell | Common Name | Standard Registry of Parts ID | Date Entered | Concentration via Spectroscopy(µg/mL) | Concentration via gel quantification (µg/mL) |
| F1 | ECFP | 470 | |||
| F2 | ECFP | 1300 | |||
| F3 | ECFP | 2010 | |||
| F4 | pBad-TetR | 400 | |||
| F5 | pBad-TetR | 575 | |||
| F6 | |||||
| F7 | |||||
| F8 | |||||
| F9 | |||||
| F10 |
Row G
| Cell | Common Name | Standard Registry of Parts ID | Date Entered | Concentration via Spectroscopy(µg/mL) | Concentration via gel quantification (µg/mL) |
| G1 | EYFP | 1300 | |||
| G2 | sRBS-Lum-dT(1) | 335 | |||
| G3 | sRBS-Lum-dT(2) | 540 | |||
| G4 | pSB-CEYFP | 340 | |||
| G5 | CFP Complete | 355 | |||
| G6 | pBad(1) | 175 | |||
| G7 | pBad(2) | 315 | |||
| G8 | N-term tag | 270 | |||
| G9 | Lumazine | 265 | |||
| G10 |
Row H
| Cell | Common Name | Standard Registry of Parts ID | Date Entered | Concentration via Spectroscopy(µg/mL) | Concentration via gel quantification (µg/mL) |
| H1 | |||||
| H2 | |||||
| H3 | |||||
| H4 | |||||
| H5 | |||||
| H6 | |||||
| H7 | |||||
| H8 | |||||
| H9 | |||||
| H10 |
Row I
| Cell | Common Name | Standard Registry of Parts ID | Date Entered | Concentration via Spectroscopy(µg/mL) | Concentration via gel quantification (µg/mL) |
| I1 | |||||
| I2 | |||||
| I3 | |||||
| I4 | |||||
| I5 | |||||
| I6 | |||||
| I7 | |||||
| I8 | |||||
| I9 | |||||
| I10 |
Row J
| Cell | Common Name | Standard Registry of Parts ID | Date Entered | Concentration via Spectroscopy(µg/mL) | Concentration via gel quantification (µg/mL) |
| J1 | |||||
| J2 | |||||
| J3 | |||||
| J4 | |||||
| J5 | |||||
| J6 | |||||
| J7 | |||||
| J8 | |||||
| J9 | |||||
| J10 |
