Team:UNIPV-Pavia/Calendar/June/settimana4
From 2010.igem.org
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<html><p align="center"><font size="4"><b>JUNE: WEEK 4</b></font></p></html><hr><br> | <html><p align="center"><font size="4"><b>JUNE: WEEK 4</b></font></p></html><hr><br> | ||
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==June, 21st== | ==June, 21st== | ||
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Other colonies resulted positives at the screening but were NOT sequenced and are stored in the ''iGEM 2010 cemetery'' box. These clones are: I1-1, I2-2, I4-1 and I6-3. | Other colonies resulted positives at the screening but were NOT sequenced and are stored in the ''iGEM 2010 cemetery'' box. These clones are: I1-1, I2-2, I4-1 and I6-3. | ||
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==June, 22nd== | ==June, 22nd== | ||
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Inoculum of I6, <partinfo>BBa_J23118</partinfo>, <partinfo>BBa_J23110</partinfo>, <partinfo>BBa_J23114</partinfo>, <partinfo>BBa_J23116</partinfo> from glycerol stock in 5ml LB+Amp. Cultures were grown ON at 37°C 220rpm. | Inoculum of I6, <partinfo>BBa_J23118</partinfo>, <partinfo>BBa_J23110</partinfo>, <partinfo>BBa_J23114</partinfo>, <partinfo>BBa_J23116</partinfo> from glycerol stock in 5ml LB+Amp. Cultures were grown ON at 37°C 220rpm. | ||
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==June, 23rd== | ==June, 23rd== | ||
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Today we received 2 stabs from iGEM HQ for <partinfo>BBa_K208001</partinfo> (one for each registry location of this part), the Silver-fusion compatible BioBrick part that codes for phasin. This part is in a pSB3K3 plasmid. Bacteria were streaked on LB+Kan (50 ug/ml) agar plates. Cultures were named PhaP-1 and PhaP-2. Plates were incubated ON at 37°C. | Today we received 2 stabs from iGEM HQ for <partinfo>BBa_K208001</partinfo> (one for each registry location of this part), the Silver-fusion compatible BioBrick part that codes for phasin. This part is in a pSB3K3 plasmid. Bacteria were streaked on LB+Kan (50 ug/ml) agar plates. Cultures were named PhaP-1 and PhaP-2. Plates were incubated ON at 37°C. | ||
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==June, 24th== | ==June, 24th== | ||
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Ligations were heated at 65°C for 5 minutes to inactivate ligase ad then tranformed in E. coli TOP10 home made competent cells. Plates were incubated ON at 37°C (we decided to incubate plates 5 hours longer than usual, because cell growth was already slower for I6). | Ligations were heated at 65°C for 5 minutes to inactivate ligase ad then tranformed in E. coli TOP10 home made competent cells. Plates were incubated ON at 37°C (we decided to incubate plates 5 hours longer than usual, because cell growth was already slower for I6). | ||
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==June, 25th== | ==June, 25th== | ||
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[[Image:Unipv_screening_PhaP_1_2.jpg|200px|thumb|center|Screening of PhaP-1 and PhaP-2]] | [[Image:Unipv_screening_PhaP_1_2.jpg|200px|thumb|center|Screening of PhaP-1 and PhaP-2]] | ||
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Latest revision as of 08:00, 31 August 2010
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