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| - | {{Team:Newcastle/mainbanner}}
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| - | '''Filamentous Cells'''
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| - | |SOS response is believe to be a universal bacteria phenomenon first studied in E.coli -LexA, recA
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| - | |In Bacillus subtillis (gram positive) dinR protein is homologous to lexA (Repressor of din-damage inducible genes).din genes include uvrA, uvrB, dinB, dinC dinR and recA. DNA damage inhibits cell division.
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| - | | Wild type Bacillus subtillis
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| - | |[[Image:Wild type Bacillus subtillis.jpg]]
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| - | |dinR KO
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| - | |[[Image:dinR KO.jpg]]
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| - | |dinR KO mutant over expressed the divergent (opposite direction) transcript for YneA, YneB and YnzC. These genes form the SOS regulon (recA independent SOS response)
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| - | | [[Image:Coding region.jpg]]
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| - | |YneA suppressed in wt without SOS induction
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| - | |Expression of YneA from ITPG controlled promoter in wt leads to elongation.
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| - | |Disruption of YneA in SOS response leads to reduced elongation. Altering YneB and YnzC expression does not affect cell morphology.
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| - | |Double mutant (dinR/YneA)
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| - | |[[Image:Double Mutant.jpg]]
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| - | |YneA protein required to suppress cell division. Not chromosome replication or segregation.
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| - | |FtsZ is important for bacterial cell division forming a ring structure at the division site by polymerising
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| - | assembling other proteins necessary for division at the site.
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| - | |FtsZ localises to the cell division cycle unless dinR is disrupted or YneA is being induced.
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| - | |YneA suppresses FtsZ ring formation- no proven direct interaction by two-hybrid.
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| - | |Filamentous cells less colony formation.
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| - | |YneA expression via the inactivation of dinR by Rec A is important.
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| - | |'''Kawai, Y., Moriya, S., & Ogasawara, N. (2003). Identification of a protein, YneA, responsible for cell division suppression during the SOS response in Bacillus subtilis. Molecular microbiology, 47(4), 1113-22. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/12581363.'''
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