Team:TU Delft/17 June 2010 content
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Today camera crew #2 came by from Nanopodium, a platform for exchanging thoughts, ideas, opinions and best practices on nanotechnology. They are making a movie about our project for the Nano Video Hunt. This is a campaign started with Hyves were young people are challenged to post videos about their views on nanotechnology. | Today camera crew #2 came by from Nanopodium, a platform for exchanging thoughts, ideas, opinions and best practices on nanotechnology. They are making a movie about our project for the Nano Video Hunt. This is a campaign started with Hyves were young people are challenged to post videos about their views on nanotechnology. | ||
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For more information look at the following websites: | For more information look at the following websites: | ||
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[http://www.nanopodium.nl www.nanopodium.nl] | [http://www.nanopodium.nl www.nanopodium.nl] | ||
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[[Image:TU_Delft_DSC4848.JPG|325px|thumb|left]] | [[Image:TU_Delft_DSC4848.JPG|325px|thumb|left]] | ||
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+ | ==Lab work== | ||
+ | By Hugo | ||
+ | |||
+ | I made some time for doing some transformations from the Biobrick plates, I will give you the list of the biobricks that I tried to transform. | ||
+ | |||
+ | {| style="color:black; background-color:white;" cellpadding="4" cellspacing="0" border="1" | ||
+ | |'''#''' | ||
+ | |'''Code''' | ||
+ | |'''Resistance marker''' | ||
+ | |'''Function''' | ||
+ | |- | ||
+ | |1 | ||
+ | |J61100 | ||
+ | |Amp | ||
+ | |RBS Anderson family | ||
+ | |- | ||
+ | |2 | ||
+ | |J61101 | ||
+ | |Amp | ||
+ | |RBS Anderson family | ||
+ | |- | ||
+ | |3 | ||
+ | |J61107 | ||
+ | |Amp | ||
+ | |RBS Anderson family | ||
+ | |- | ||
+ | |4 | ||
+ | |J61117 | ||
+ | |Amp | ||
+ | |RBS Anderson family | ||
+ | |- | ||
+ | |5 | ||
+ | |J61127 | ||
+ | |Amp | ||
+ | |RBS Anderson family | ||
+ | |- | ||
+ | |6 | ||
+ | |B0034 | ||
+ | |Amp | ||
+ | |RBS | ||
+ | |- | ||
+ | |7 | ||
+ | |J23109 | ||
+ | |Amp | ||
+ | |Promoter Anderson family | ||
+ | |- | ||
+ | |8 | ||
+ | |B0032 | ||
+ | |Amp | ||
+ | |RBS Anderson family | ||
+ | |- | ||
+ | |9 | ||
+ | |R0011 | ||
+ | |Amp | ||
+ | |LacI promoter | ||
+ | |- | ||
+ | |10 | ||
+ | |B0015 | ||
+ | |Amp, Kan | ||
+ | |Double terminator | ||
+ | |- | ||
+ | |11 | ||
+ | |R0010 | ||
+ | |Amp | ||
+ | |LacI | ||
+ | |- | ||
+ | |12 | ||
+ | |E0422 | ||
+ | |Amp | ||
+ | |YFP generator | ||
+ | |- | ||
+ | |13 | ||
+ | |E0240 | ||
+ | |Amp | ||
+ | |GFP generator | ||
+ | |- | ||
+ | |14 | ||
+ | |E0040 | ||
+ | |Amp | ||
+ | |GFP | ||
+ | |- | ||
+ | |15 | ||
+ | |I13401 | ||
+ | |Amp | ||
+ | |GFP + Double terminator | ||
+ | |} | ||
+ | |||
+ | |||
+ | Procedure: | ||
+ | * Add 1 ul of DNA to 33 ul of competent cells | ||
+ | * Put the tubes on ice for 10 min | ||
+ | * Warm the tubes in a water bath at 37º C for 5 min. | ||
+ | * Add 250 ul of LB medium | ||
+ | * Incubate the cells 1 hour at 37ºC, shake at 160 rpm | ||
+ | * Plate 200 ul over selective medium |
Latest revision as of 16:01, 25 August 2010
More media
Today camera crew #2 came by from Nanopodium, a platform for exchanging thoughts, ideas, opinions and best practices on nanotechnology. They are making a movie about our project for the Nano Video Hunt. This is a campaign started with Hyves were young people are challenged to post videos about their views on nanotechnology.
For more information look at the following websites:
[http://www.nanovideohunt.nl www.nanovideohunt.nl]
[http://www.nanovideohunt.hyves.nl www.nanovideohunt.hyves.nl]
[http://www.nanopodium.nl www.nanopodium.nl]
Lab work
By Hugo
I made some time for doing some transformations from the Biobrick plates, I will give you the list of the biobricks that I tried to transform.
# | Code | Resistance marker | Function |
1 | J61100 | Amp | RBS Anderson family |
2 | J61101 | Amp | RBS Anderson family |
3 | J61107 | Amp | RBS Anderson family |
4 | J61117 | Amp | RBS Anderson family |
5 | J61127 | Amp | RBS Anderson family |
6 | B0034 | Amp | RBS |
7 | J23109 | Amp | Promoter Anderson family |
8 | B0032 | Amp | RBS Anderson family |
9 | R0011 | Amp | LacI promoter |
10 | B0015 | Amp, Kan | Double terminator |
11 | R0010 | Amp | LacI |
12 | E0422 | Amp | YFP generator |
13 | E0240 | Amp | GFP generator |
14 | E0040 | Amp | GFP |
15 | I13401 | Amp | GFP + Double terminator |
Procedure:
- Add 1 ul of DNA to 33 ul of competent cells
- Put the tubes on ice for 10 min
- Warm the tubes in a water bath at 37º C for 5 min.
- Add 250 ul of LB medium
- Incubate the cells 1 hour at 37ºC, shake at 160 rpm
- Plate 200 ul over selective medium