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Team:Cambridge/Notebook/7
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== Monday== | == Monday== | ||
| - | Today we | + | Today the whole team was in the lab at the same time - it was very exciting. |
| + | |||
| + | Now that we have all our oligos, Anja and Will have been assembling the lux operon with a pBAD promoter from V. fischeri using Gibson. They set a race going between the 3 different PCR machines but unfortunately they all failed for different reasons. | ||
| + | |||
| + | Paul has been working on the firefly luciferase from the registry, performing a colony PCR in order to isolate the promoter and luciferase from previous experiments to make the biobrick for submission. | ||
| + | |||
| + | Peter, Ben and Emily (who was now back from Wales) worked on the plate reader experiment that Ben and Peter had planned last week. They plated out some Top10 glycerol stocks of the promoter, rbs and registry luciferase to make liquid culture tomorrow and start the plate reader experiments on Wednesday. | ||
| + | |||
| + | Hannah and Theo also transformed the pJS555 plasmid from Jim Slock, which doesn't contain the phage DNA present in pHK555. Bill continued with his amazing python program for designing primers. | ||
Revision as of 15:43, 23 August 2010
Contents |
Monday
Today the whole team was in the lab at the same time - it was very exciting.
Now that we have all our oligos, Anja and Will have been assembling the lux operon with a pBAD promoter from V. fischeri using Gibson. They set a race going between the 3 different PCR machines but unfortunately they all failed for different reasons.
Paul has been working on the firefly luciferase from the registry, performing a colony PCR in order to isolate the promoter and luciferase from previous experiments to make the biobrick for submission.
Peter, Ben and Emily (who was now back from Wales) worked on the plate reader experiment that Ben and Peter had planned last week. They plated out some Top10 glycerol stocks of the promoter, rbs and registry luciferase to make liquid culture tomorrow and start the plate reader experiments on Wednesday.
Hannah and Theo also transformed the pJS555 plasmid from Jim Slock, which doesn't contain the phage DNA present in pHK555. Bill continued with his amazing python program for designing primers.
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
