Team:LMU-Munich/Jump-or-die/Schedule

(Difference between revisions)

Revision as of 16:23, 20 August 2010


	Cut'N'Survive System Functional Principle Sequences Schedule Jump-or-Die System Functional Principle Sequences Schedule ProSearch System Schedule

	Functional Principle Sequences Schedule picture gallery

For PCR key see PCR key

Colourcode for the primers: Annealing part, mutagenised part, other sequences integrated into primer

PCR1: replication of the Tet-inducible CMV promotor [X]

Primer used: 1TreF 2TreR expected product size: 492 bp

PCR4a: replication human bak with mutagenesis (Pst1) [ ]

Primer used: 7BakF 8BakMutPR expected product size: 330 bp

PCR4b: replication human bak with mutagenesis (Pst1) [ ]

Primer used: 9BakMutPF 10BakR expected product size: 376 bp

PCR5: joining PCR of human bak [ ]

Primer used: 7BakF 10BakR expected product size: 688 bp

PCR6: replication of the SV40-polyadenylation site [X]

Primer used: 11PAF 12PAR expected product size: 237 bp

PCR9: replication of eGFP with attP in primer [ ]

Primer used: 20eGFPattPF 21eGFPR expected product size: 808 bp

PCR10: Replikation of PhiC31o [ ]

Primer used: 22PC31oF 23PC31oR expected product size: 1888 bp

Note: for attB two Primers ( 18attBF 19attBR) used.

We are using the 3A System to assemble Biobricks.

Assembling Construct 1

Assembling Construct 2

Assembling Construct 3

@@ Line 60: / Line 60: @@
 ==Aim 2: Assembling Biobricks==
-We are using the 3A System to assemble Biobricks.
+We are using the [[Team:LMU-Munich/Notebook/Protocols/13_3A_Method_for_Biobrick_assembly|3A System]] to assemble Biobricks.
 <b> Assembling Construct 1</b>