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Team:LMU-Munich/Schedule
From 2010.igem.org
(→PCR and mutagenisis of the digestion sites) |
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==PCR and mutagenisis of the digestion sites== | ==PCR and mutagenisis of the digestion sites== | ||
| - | PCR1a: replication of Pdu D with | + | <b>PCR1a: replication of Pdu D with mutagenesis [ ] </b> |
[[Image:PCR1aP.jpg]] | [[Image:PCR1aP.jpg]] | ||
| + | expected product size: bp | ||
| - | PCR1b: replication of Pdu D with | + | |
| + | <b>PCR1b: replication of Pdu D with mutagenesis [ ] </b> | ||
[[Image:PCR1bP.jpg]] | [[Image:PCR1bP.jpg]] | ||
| + | expected product size: bp | ||
| - | PCR2: Joining PCR of PduD | + | |
| + | <b>PCR2: Joining PCR of PduD [ ] </b> | ||
[[Image:PCR2P.jpg]] | [[Image:PCR2P.jpg]] | ||
| + | expected product size: bp | ||
| + | |||
| - | PCR3a: replication of Pdu C with | + | <b>PCR3a: replication of Pdu C with mutagenesis [ ] </b> |
[[Image:PCR3aP.jpg]] | [[Image:PCR3aP.jpg]] | ||
| + | expected product size: bp | ||
| - | PCR3b: replication of Pdu C with | + | |
| + | <b>PCR3b: replication of Pdu C with mutagenesis [ ] </b> | ||
[[Image:PCR3bP.jpg]] | [[Image:PCR3bP.jpg]] | ||
| + | expected product size: bp | ||
| - | PCR4: Joining PCR of Pdu C | + | |
| + | <b>PCR4: Joining PCR of Pdu C [ ] </b> | ||
[[Image:PCR4P.jpg]] | [[Image:PCR4P.jpg]] | ||
| + | expected product size: bp | ||
| + | |||
| - | PCR5: replication of AurF to be joined with Pdu D | + | <b>PCR5: replication of AurF to be joined with Pdu D [ ] </b> |
[[Image:PCR5P.jpg]] | [[Image:PCR5P.jpg]] | ||
| + | expected product size: bp | ||
| - | PCR6a: replication of LctO with | + | |
| + | <b>PCR6a: replication of LctO with mutagenesis [ ] </b> | ||
[[Image:PCR6aP.jpg]] | [[Image:PCR6aP.jpg]] | ||
| + | expected product size: bp | ||
| - | PCR6b: replication of LctO with | + | |
| + | <b>PCR6b: replication of LctO with mutagenesis [ ] </b> | ||
[[Image:PCR6bP.jpg]] | [[Image:PCR6bP.jpg]] | ||
| + | expected product size: bp | ||
| + | |||
| - | PCR7: Joining PCR of LctO | + | <b>PCR7: Joining PCR of LctO [ ] </b> |
[[Image: PCR7P.jpg]] | [[Image: PCR7P.jpg]] | ||
| + | expected product size: bp | ||
| - | PCR8a: replication of LctO with | + | |
| + | <b>PCR8a: replication of LctO with mutagenesis [ ] </b> | ||
[[Image:PCR8aP.jpg]] | [[Image:PCR8aP.jpg]] | ||
| + | expected product size: bp | ||
| + | |||
| - | PCR8b: replication of LctO with | + | <b>PCR8b: replication of LctO with mutagenesis [ ] </b> |
[[Image:PCR8bP.jpg]] | [[Image:PCR8bP.jpg]] | ||
| + | expected product size: bp | ||
| - | PCR9: Joining PCR of LctO | + | |
| + | <b>PCR9: Joining PCR of LctO [ ] </b> | ||
[[Image:PCR9P.jpg]] | [[Image:PCR9P.jpg]] | ||
| + | expected product size: bp | ||
| - | PCR10: Joining PCR of AurF and PduD | + | |
| + | <b>PCR10: Joining PCR of AurF and PduD [ ] </b> | ||
[[Image:PCR10P.jpg]] | [[Image:PCR10P.jpg]] | ||
| + | expected product size: bp | ||
| + | |||
| - | PCR11: Joining PCR of LctO and PduC | + | <b>PCR11: Joining PCR of LctO and PduC [ ] </b> |
[[Image:PCR11P.jpg]] | [[Image:PCR11P.jpg]] | ||
| + | expected product size: bp | ||
| - | PCR12a: replication of the Pdu-Operon with | + | |
| + | <b>PCR12a: replication of the Pdu-Operon with mutagenesis [ ] </b> | ||
[[Image:PCR12aP.jpg]] | [[Image:PCR12aP.jpg]] | ||
| + | expected product size: bp | ||
| - | PCR12b: replication of the Pdu-Operon with | + | |
| + | <b>PCR12b: replication of the Pdu-Operon with mutagenesis [ ] </b> | ||
[[Image:PCR12bP.jpg]] | [[Image:PCR12bP.jpg]] | ||
| + | expected product size: bp | ||
| + | |||
| - | PCR13: Joining PCR of the Pdu-Operon | + | <b>PCR13: Joining PCR of the Pdu-Operon [ ] </b> |
[[Image:PCR131517P.jpg]] | [[Image:PCR131517P.jpg]] | ||
| + | expected product size: bp | ||
| - | PCR14a: replication of the Pdu-Operon with | + | |
| + | <b>PCR14a: replication of the Pdu-Operon with mutagenesis [ ] </b> | ||
[[Image:PCR14aP.jpg]] | [[Image:PCR14aP.jpg]] | ||
| + | expected product size: bp | ||
| + | |||
| - | PCR14b: replication of the Pdu-Operon with | + | <b>PCR14b: replication of the Pdu-Operon with mutagenesis [ ] </b> |
[[Image:PCR14bP.jpg]] | [[Image:PCR14bP.jpg]] | ||
| + | expected product size: bp | ||
| - | PCR15: Joining PCR of the Pdu-Operon | + | |
| + | <b>PCR15: Joining PCR of the Pdu-Operon [ ] </b> | ||
[[Image:PCR131517P.jpg]] | [[Image:PCR131517P.jpg]] | ||
| + | expected product size: bp | ||
| - | PCR16a: replication of the Pdu-Operon with | + | |
| + | <b>PCR16a: replication of the Pdu-Operon with mutagenesis [ ] </b> | ||
[[Image:PCR16aP.jpg]] | [[Image:PCR16aP.jpg]] | ||
| + | expected product size: bp | ||
| - | PCR16b: replication of the Pdu-Operon with | + | |
| + | <b>PCR16b: replication of the Pdu-Operon with mutagenesis [ ] </b> | ||
[[Image:PCR16bP.jpg]] | [[Image:PCR16bP.jpg]] | ||
| + | expected product size: bp | ||
| + | |||
| - | PCR17: Joining PCR of the Pdu-Operon | + | <b>PCR17: Joining PCR of the Pdu-Operon |
[[Image:PCR131517P.jpg]] | [[Image:PCR131517P.jpg]] | ||
| + | expected product size: bp | ||
==In Vitro testing of the chemical kinetics with the enzymes== | ==In Vitro testing of the chemical kinetics with the enzymes== | ||
Revision as of 11:53, 20 August 2010
PCR1a: replication of Pdu D with mutagenesis [ ]
Contents
PCR and mutagenisis of the digestion sites
expected product size: bp
PCR1b: replication of Pdu D with mutagenesis [ ]
expected product size: bp
PCR2: Joining PCR of PduD [ ]
expected product size: bp
PCR3a: replication of Pdu C with mutagenesis [ ]
expected product size: bp
PCR3b: replication of Pdu C with mutagenesis [ ]
expected product size: bp
PCR4: Joining PCR of Pdu C [ ]
expected product size: bp
PCR5: replication of AurF to be joined with Pdu D [ ]
expected product size: bp
PCR6a: replication of LctO with mutagenesis [ ]
expected product size: bp
PCR6b: replication of LctO with mutagenesis [ ]
expected product size: bp
PCR7: Joining PCR of LctO [ ]
expected product size: bp
PCR8a: replication of LctO with mutagenesis [ ]
expected product size: bp
PCR8b: replication of LctO with mutagenesis [ ]
expected product size: bp
PCR9: Joining PCR of LctO [ ]
expected product size: bp
PCR10: Joining PCR of AurF and PduD [ ]
expected product size: bp
PCR11: Joining PCR of LctO and PduC [ ]
expected product size: bp
PCR12a: replication of the Pdu-Operon with mutagenesis [ ]
expected product size: bp
PCR12b: replication of the Pdu-Operon with mutagenesis [ ]
expected product size: bp
PCR13: Joining PCR of the Pdu-Operon [ ]
expected product size: bp
PCR14a: replication of the Pdu-Operon with mutagenesis [ ]
expected product size: bp
PCR14b: replication of the Pdu-Operon with mutagenesis [ ]
expected product size: bp
PCR15: Joining PCR of the Pdu-Operon [ ]
expected product size: bp
PCR16a: replication of the Pdu-Operon with mutagenesis [ ]
expected product size: bp
PCR16b: replication of the Pdu-Operon with mutagenesis [ ]
expected product size: bp
PCR17: Joining PCR of the Pdu-Operon
expected product size: bp
In Vitro testing of the chemical kinetics with the enzymes
Transforming the construct in bacteria
Testing the system
