Team:LMU-Munich/Cut'N'survive/Schedule

From 2010.igem.org

(Difference between revisions)

Revision as of 11:29, 20 August 2010


	Cut'N'Survive System Functional Principle Sequences Schedule Jump-or-Die System Functional Principle Sequences Schedule ProSearch System Schedule

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Aim 1: DNA replication+PCR

For PCR key see PCR key

Colourcode for the primers: Annealing part, mutagenised part, other sequences integrated into primer

PCR1: replication of the Tet-inducible CMV promotor [X]

Primer used: 1TreF 2TreR expected product size: 492bp

PCR2a: replication of the TEVrecogn-NDegron-SF3 Part with mutagenisis (EcoR1 + Pst1) [X]

Primer used: 3TevF 4TevMutEPR expected product size: bp

PCR2b: replication of the TEVrecogn-NDegron-SF3 Part with mutagenisis (EcoR1 + Pst1) [X]

Primer used: 5TevMutEPF 6TevR expected product size: bp

PCR3: joining PCR of the TEVrecogn-NDegron-SF3 Part [ ]

Primer used: 3TevF 6TevR expected product size: bp

PCR4a: replication human bak with mutagenisis (Pst1) [ ]

Primer used: 7BakF 8BakMutPR expected product size: bp

PCR4b: replication human bak with mutagenisis (Pst1) [ ]

Primer used: 9BakMutPF 10BakR expected product size: bp

PCR5: joining PCR of human bak [ ]

Primer used: 7BakF 10BakR expected product size: 685 bp

PCR6: replication of the SV40-polyadenylation site [X]

Primer used: 11PAF 12PAR expected product size: bp

PCR7a: replication of the p14*TEVrecogn with mutagenisis (Spe1) [ ]

Primer used: 13TrecF 14TrecMutSR expected product size: bp

PCR7b: replication of the p14*TEVrecogn with mutagenisis (Spe1) with TEV recogn in primer (16TrecR) [ ]

Primer used: 15TrecMutSF 16TrecR expected product size: bp

PCR8: joining PCR of p14*TEVrecogn with TEV recogn in primer (16TrecR) [ ]

Primer used: 13TrecF 16TrecR expected product size: bp

@@ Line 11: / Line 11: @@
 [[Image:PCR1.jpg]]
-Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer1| 1TreF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer2| 2TreR]] expected productsize: 492bp
+Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer1| 1TreF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer2| 2TreR]]
+expected product size: 492bp
@@ Line 18: / Line 19: @@
 Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer3| 3TevF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer4| 4TevMutEPR]]
+expected product size: bp
@@ Line 24: / Line 26: @@
 Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer5| 5TevMutEPF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer6| 6TevR]]
+expected product size: bp
@@ Line 30: / Line 33: @@
 Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer3| 3TevF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer6| 6TevR]]
+expected product size: bp
@@ Line 36: / Line 40: @@
 Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer7| 7BakF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer8| 8BakMutPR]]
+expected product size: bp
@@ Line 42: / Line 47: @@
 Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer9| 9BakMutPF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer10| 10BakR]]
+expected product size: bp
@@ Line 55: / Line 61: @@
 Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer11| 11PAF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer12| 12PAR]]
+expected product size: bp
@@ Line 61: / Line 68: @@
 Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer13| 13TrecF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer14| 14TrecMutSR]]
+expected product size: bp
@@ Line 67: / Line 75: @@
 Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer15| 15TrecMutSF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer16| 16TrecR]]
+expected product size: bp
@@ Line 73: / Line 82: @@
 Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer13| 13TrecF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer16| 16TrecR]]
+expected product size: bp
 ==Aim 2: Assembling Biobricks==

Team:LMU-Munich/Cut'N'survive/Schedule

From 2010.igem.org

Revision as of 11:29, 20 August 2010

Contents

Aim 1: DNA replication+PCR

Aim 2: Assembling Biobricks

Aim 3: Testing products

Aim 4: Testing system