Team:LMU-Munich/Cut'N'survive/Schedule

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(Difference between revisions)

Revision as of 11:27, 20 August 2010


	Cut'N'Survive System Functional Principle Sequences Schedule Jump-or-Die System Functional Principle Sequences Schedule ProSearch System Schedule

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Aim 1: DNA replication+PCR

For PCR key see PCR key

Colourcode for the primers: Annealing part, mutagenised part, other sequences integrated into primer

PCR1: replication of the Tet-inducible CMV promotor [X]

Primer used: 1TreF 2TreR expected productsize: 492bp

PCR2a: replication of the TEVrecogn-NDegron-SF3 Part with mutagenisis (EcoR1 + Pst1) [X]

Primer used: 3TevF 4TevMutEPR

PCR2b: replication of the TEVrecogn-NDegron-SF3 Part with mutagenisis (EcoR1 + Pst1) [X]

Primer used: 5TevMutEPF 6TevR

PCR3: joining PCR of the TEVrecogn-NDegron-SF3 Part [ ]

Primer used: 3TevF 6TevR

PCR4a: replication human bak with mutagenisis (Pst1) [ ]

Primer used: 7BakF 8BakMutPR

PCR4b: replication human bak with mutagenisis (Pst1) [ ]

Primer used: 9BakMutPF 10BakR

PCR5: joining PCR of human bak [ ]

Primer used: 7BakF 10BakR expected product size: 685 bp

PCR6: replication of the SV40-polyadenylation site [X]

Primer used: 11PAF 12PAR

PCR7a: replication of the p14*TEVrecogn with mutagenisis (Spe1) [ ]

Primer used: 13TrecF 14TrecMutSR

PCR7b: replication of the p14*TEVrecogn with mutagenisis (Spe1) with TEV recogn in primer (16TrecR) [ ]

Primer used: 15TrecMutSF 16TrecR

PCR8: joining PCR of p14*TEVrecogn with TEV recogn in primer (16TrecR) [ ]

Primer used: 13TrecF 16TrecR

@@ Line 6: / Line 6: @@
 <b>Colourcode for the primers: <font color="#FF0000">Annealing part</font>, <font color="#009933">mutagenised part</font>, <font color="#FF6600">other sequences integrated into primer</font></b>
 <b>PCR1: replication of the Tet-inducible CMV promotor   [X]</b>
@@ Line 12: / Line 13: @@
 Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer1| 1TreF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer2| 2TreR]] expected productsize: 492bp
-PCR2a: replication of the TEVrecogn-NDegron-SF3 Part with mutagenisis (EcoR1 + Pst1) [X]
+<b>PCR2a: replication of the TEVrecogn-NDegron-SF3 Part with mutagenisis (EcoR1 + Pst1) [X]</b>
 [[Image:PCR2a.jpg]]
 Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer3| 3TevF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer4| 4TevMutEPR]]
-PCR2b: replication of the TEVrecogn-NDegron-SF3 Part with mutagenisis (EcoR1 + Pst1) [X]
+<b>PCR2b: replication of the TEVrecogn-NDegron-SF3 Part with mutagenisis (EcoR1 + Pst1) [X]</b>
 [[Image:PCR2b.jpg]]
 Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer5| 5TevMutEPF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer6| 6TevR]]
-PCR3: joining PCR of the TEVrecogn-NDegron-SF3 Part [ ]
+<b>PCR3: joining PCR of the TEVrecogn-NDegron-SF3 Part [ ]</b>
 [[Image:PCR3.jpg]]
 Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer3| 3TevF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer6| 6TevR]]
-PCR4a: replication human bak with mutagenisis (Pst1) [ ]
+<b>PCR4a: replication human bak with mutagenisis (Pst1) [ ]</b>
 [[Image:PCR4a.jpg]]
 Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer7| 7BakF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer8| 8BakMutPR]]
-PCR4b: replication human bak with mutagenisis (Pst1) [ ]
+<b>PCR4b: replication human bak with mutagenisis (Pst1) [ ]</b>
 [[Image:PCR4b.jpg]]
 Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer9| 9BakMutPF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer10| 10BakR]]
-PCR5: joining PCR of human bak [ ]
+<b>PCR5: joining PCR of human bak [ ]</b>
 [[Image:PCR5.jpg]]
 Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer7| 7BakF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer10| 10BakR]]
 expected product size: 685 bp
-PCR6: replication of the SV40-polyadenylation site [X]
+<b>PCR6: replication of the SV40-polyadenylation site [X]</b>
 [[Image:PCR6.jpg]]
 Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer11| 11PAF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer12| 12PAR]]
-PCR7a: replication of the p14*TEVrecogn with mutagenisis (Spe1) [ ]
+<b>PCR7a: replication of the p14*TEVrecogn with mutagenisis (Spe1) [ ]</b>
 [[Image:PCR7a.jpg]]
 Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer13| 13TrecF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer14| 14TrecMutSR]]
-PCR7b: replication of the p14*TEVrecogn with mutagenisis (Spe1) with TEV recogn in primer (16TrecR) [ ]
+<b>PCR7b: replication of the p14*TEVrecogn with mutagenisis (Spe1) with TEV recogn in primer (16TrecR) [ ]</b>
 [[Image:PCR7b.jpg]]
 Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer15| 15TrecMutSF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer16| 16TrecR]]
-PCR8: joining PCR of p14*TEVrecogn with TEV recogn in primer (16TrecR) [ ]
+<b>PCR8: joining PCR of p14*TEVrecogn with TEV recogn in primer (16TrecR) [ ]</b>
 [[Image:PCR8.jpg]]

Team:LMU-Munich/Cut'N'survive/Schedule

From 2010.igem.org

Revision as of 11:27, 20 August 2010

Contents

Aim 1: DNA replication+PCR

Aim 2: Assembling Biobricks

Aim 3: Testing products

Aim 4: Testing system