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Team:HKU-Hong Kong/Project
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=== Killing Mechanism === | === Killing Mechanism === | ||
| + | *Enterobacteriophage T4 Lysis Device | ||
| + | **We found the biobrick form Berkeley's team from iGEM 2008 a possible way to attain cell destruction. | ||
| + | [[Image:T4mec.JPG|500px]] | ||
| - | + | Uses genes from enterobacteriaphage T4 (endolysin – lysozyme, holin and antiholin). Various promoters can be installed into this device to control lysis. Antiholin has its own constitutive promoter to prevent formation of holin multimers from basal expression. When device is off, higher expression of antiholin is needed for better stability of the device. | |
| - | + | ||
=== Regulation === | === Regulation === | ||
Revision as of 05:02, 20 August 2010
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Several ideas on the subject of this year's investigation were come up during various brainstorming sessions. Our team picked out the idea of engineering a mechanism that can act as a safety net to prevent genetically modified bacteria from performing undesired tasks in wrong environments.
Bacteria are genetically engineered to perform various functions.Prospective functions include biodegradation of crude oil and killing of cancer cells. Yet, undesired tasks might be performed by the baterium itself as well. The idea of a "bio-safety" net would serve the purpose.
For example, after fulfillment of intended functions or when bacteria have escaped from the intented work zone.
File:HKU-Hong Kong team.png Your team picture |
Contents |
Overall project
ways to achieve this
- Insertion of killing genes
- Regulated by specific promoters
- Promoters respond to changes in the environment
Hence bacteria can and can only function well and survive in a specific environment
Project Details
Killing Mechanism
- Enterobacteriophage T4 Lysis Device
- We found the biobrick form Berkeley's team from iGEM 2008 a possible way to attain cell destruction.
Uses genes from enterobacteriaphage T4 (endolysin – lysozyme, holin and antiholin). Various promoters can be installed into this device to control lysis. Antiholin has its own constitutive promoter to prevent formation of holin multimers from basal expression. When device is off, higher expression of antiholin is needed for better stability of the device.
