Team:LMU-Munich/Cut'N'survive/Schedule

(Difference between revisions)

Revision as of 14:05, 19 August 2010


	Cut'N'Survive System Functional Principle Sequences Schedule Jump-or-Die System Functional Principle Sequences Schedule ProSearch System Schedule

	Functional Principle Sequences Schedule picture gallery

For PCR key see PCR key

Colourcode for the primers: Annealing part, mutagenised part, other sequences integrated into primer

PCR1: replication of the Tet-inducible CMV promotor [X]

Primer used: 1TreF 2TreR

PCR2a: replication of the TEVrecogn-NDegron-SF3 Part with mutagenisis (EcoR1 + Pst1) [X]

PCR2b: replication of the TEVrecogn-NDegron-SF3 Part with mutagenisis (EcoR1 + Pst1) [X]

PCR3: joining PCR of the TEVrecogn-NDegron-SF3 Part [ ]

Primer used: 3TevF 6TevR

PCR4a: replication human bak with mutagenisis (Pst1) [ ]

PCR4b: replication human bak with mutagenisis (Pst1) [ ]

PCR5: joining PCR of human bak [ ]

Primer used: 7BakF 10BakR

PCR6: replication of the SV40-polyadenylation site [X]

Primer used: 11PAF 12PAR

PCR7a: replication of the p14*TEVrecogn with mutagenisis (Spe1) [ ]

PCR7b: replication of the p14*TEVrecogn with mutagenisis (Spe1) with TEV recogn in primer (16TrecR) [ ]

PCR8: joining PCR of p14*TEVrecogn with TEV recogn in primer (16TrecR) [ ]

@@ Line 12: / Line 12: @@
 Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer1| 1TreF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer2| 2TreR]]
-PCR2a: replication of the TEVrecogn-NDegron-SF3 Part with mutagenisis (EcoR1 + Pst1) [ ]
+PCR2a: replication of the TEVrecogn-NDegron-SF3 Part with mutagenisis (EcoR1 + Pst1) [X]
 [[Image:PCR2a.jpg]]
 Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer3| 3TevF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer4| 4TevMutEPR]]
-PCR2b: replication of the TEVrecogn-NDegron-SF3 Part with mutagenisis (EcoR1 + Pst1) [ ]
+PCR2b: replication of the TEVrecogn-NDegron-SF3 Part with mutagenisis (EcoR1 + Pst1) [X]
 [[Image:PCR2b.jpg]]