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Team:LMU-Munich/Cut'N'survive/Schedule/PCR key
From 2010.igem.org
(Difference between revisions)
(New page: {{:Team:LMU-Munich/Templates/Page Header}} {| |- |1TreF 56°C: |#Number: consecutive numbering on PCR plan |- | |#Tre: Abbreviation of gene of interest |- | |#F: Forward Primer |- | |#56°...) |
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{{:Team:LMU-Munich/Templates/Page Header}} | {{:Team:LMU-Munich/Templates/Page Header}} | ||
| + | ==PCR Key== | ||
| + | |||
{| | {| | ||
|- | |- | ||
|1TreF 56°C: | |1TreF 56°C: | ||
| - | | | + | |*Number: consecutive numbering on PCR plan |
|- | |- | ||
| | | | ||
| - | | | + | |*Tre: Abbreviation of gene of interest |
|- | |- | ||
| | | | ||
| - | | | + | |*F: Forward Primer |
|- | |- | ||
| | | | ||
| - | | | + | |*56°C: Annealing temperature (4+2 rule) |
|- | |- | ||
|4TevMutEPR : | |4TevMutEPR : | ||
| - | | | + | |*Mut: Mutagenesis Primer |
|- | |- | ||
| | | | ||
| - | | | + | |*E: EcoRI digestion site |
|- | |- | ||
| | | | ||
| - | | | + | |*P: PstI digestion site |
|- | |- | ||
| | | | ||
| - | | | + | |*R: Reverse Primer |
|- | |- | ||
|Also: | |Also: | ||
| - | | | + | |*S: SpeI digestion site |
|} | |} | ||
| + | |||
| + | <html> | ||
| + | <table> | ||
| + | <tr> | ||
| + | <td> | ||
| + | <img src="https://static.igem.org/mediawiki/2010/7/7f/Primer.jpg" width="89" height="15" border="0"/> | ||
| + | </td> | ||
| + | <td> | ||
| + | Primer | ||
| + | </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td> | ||
| + | <img src="https://static.igem.org/mediawiki/2010/6/6e/Overhang.jpg" width="51" height="15" border="0"/> | ||
| + | </td> | ||
| + | <td> | ||
| + | Primer overhang with Biobrick digestion sites | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td> | ||
| + | <img src="https://static.igem.org/mediawiki/2010/0/0a/Mutation.jpg" width="32" height="30" border="0"/> | ||
| + | </td> | ||
| + | <td> | ||
| + | Digestion site which is to be mutated | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td> | ||
| + | <img src="https://static.igem.org/mediawiki/2010/e/e4/Template.jpg" width="125" height="9" border="0"/> | ||
| + | </td> | ||
| + | <td> | ||
| + | Template | ||
| + | </td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | </html> | ||
{{:Team:LMU-Munich/Templates/Page Footer}} | {{:Team:LMU-Munich/Templates/Page Footer}} | ||
Latest revision as of 15:11, 18 August 2010
PCR Key
1TreF 56°C:
*Number: consecutive numbering on PCR plan
*Tre: Abbreviation of gene of interest
*F: Forward Primer
*56°C: Annealing temperature (4+2 rule)
4TevMutEPR :
*Mut: Mutagenesis Primer
*E: EcoRI digestion site
*P: PstI digestion site
*R: Reverse Primer
Also:
*S: SpeI digestion site
Primer
Primer overhang with Biobrick digestion sites
Digestion site which is to be mutated
Template
