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Team:LMU-Munich/Jump-or-die/Schedule
From 2010.igem.org
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==Aim 1: DNA reproduction+PCR== | ==Aim 1: DNA reproduction+PCR== | ||
| + | <b>Colourcode for the primers: <font color="#FF0000">Annealing part</font>, <font color="#009933">mutagenised part</font>, <font color="#FF6600">other sequences integrated into primer</font></b> | ||
| + | |||
PCR1: replication of the Tet-inducible CMV promotor [X] | PCR1: replication of the Tet-inducible CMV promotor [X] | ||
[[Image:PCR1.jpg]] | [[Image:PCR1.jpg]] | ||
Revision as of 13:38, 18 August 2010
Colourcode for the primers: Annealing part, mutagenised part, other sequences integrated into primer
PCR1: replication of the Tet-inducible CMV promotor [X]
PCR4a: replication human bak with mutagenesis (Pst1) [ ]
PCR4b: replication human bak with mutagenesis (Pst1) [ ]
PCR5: joining PCR of human bak [ ]
PCR6: replication of the SV40-polyadenylation site [X]
PCR9: replication of eGFP with attP in primer [ ]
Primer used: 20eGFPattPF 21eGFPR
PCR10: Replikation of PhiC31o [ ]
Primer used: 22PC31oF 23PC31oR
Note: for attB two Primers ( 18attBF 19attBR) used.
Contents
Aim 1: DNA reproduction+PCR
Aim 2: Assembling Biobricks
Aim 3: Testing products
Aim 4: Testing system
