Team:LMU-Munich/Jump-or-die/Schedule

(Difference between revisions)

Revision as of 13:37, 18 August 2010


	Cut'N'Survive System Functional Principle Sequences Schedule Jump-or-Die System Functional Principle Sequences Schedule ProSearch System Schedule

	Functional Principle Sequences Schedule picture gallery

PCR1: replication of the Tet-inducible CMV promotor [X]

Primer used: 1TreF 2TreR

PCR4a: replication human bak with mutagenesis (Pst1) [ ]

PCR4b: replication human bak with mutagenesis (Pst1) [ ]

PCR5: joining PCR of human bak [ ]

Primer used: 7BakF 10BakR

PCR6: replication of the SV40-polyadenylation site [X]

Primer used: 11PAF 12PAR

PCR9: replication of eGFP with attP in primer [ ]

PCR10: Replikation of PhiC31o [ ]

Note: for attB two Primers ( 18attBF 19attBR) used.

@@ Line 7: / Line 7: @@
 Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer1| 1TreF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer2| 2TreR]]
-PCR4a: replication human bak with mutagenisis (Pst1) [ ]
+PCR4a: replication human bak with mutagenesis (Pst1) [ ]
 [[Image:PCR4a.jpg]]
 Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer7| 7BakF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer8| 8BakMutPR]]
-PCR4b: replication human bak with mutagenisis (Pst1) [ ]
+PCR4b: replication human bak with mutagenesis (Pst1) [ ]
 [[Image:PCR4b.jpg]]