Team:LMU-Munich/Protocoll

From 2010.igem.org

(Difference between revisions)
(New page: ==PCR master mix== MQ 34,2 10 x Buffer 5 pF 3 pR 3 Template 2 dNTPs 2 Pfu (Promega) 0,8 final volume 50 µl)
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==PCR master mix==
+
=='''PCR master mix'''==
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MQ         34,2
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* MQ         34,2
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10 x Buffer 5
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* 10 x Buffer 5
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pF         3
+
* pF         3
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pR         3
+
* pR         3
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Template 2
+
* Template 2
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dNTPs         2
+
* dNTPs         2
-
Pfu (Promega) 0,8
+
* Pfu (Promega) 0,8
-
final volume 50 µl
+
* final volume 50 µl

Revision as of 09:41, 17 August 2010

PCR master mix

  • MQ 34,2
  • 10 x Buffer 5
  • pF 3
  • pR 3
  • Template 2
  • dNTPs 2
  • Pfu (Promega) 0,8
  • final volume 50 µl