Team:UNIPV-Pavia/Calendar/August/settimana3

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(August, 16th)
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Transformation of 15-4C5 (1 ul) into in recompetentized ''E. coli'' TOP10 carrying <partinfo>BBa_T9002</partinfo>.
Transformation of 15-4C5 (1 ul) into in recompetentized ''E. coli'' TOP10 carrying <partinfo>BBa_T9002</partinfo>.
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Transfomants were plated on LB+Amp 100 ug/ml agar plates, the last one on LB+Amp100+Cm12,5 agar plates and were let grow ON at 37°C.
==August, 17th==
==August, 17th==

Revision as of 15:00, 16 August 2010


AUGUST: WEEK 2



August, 16th

Resuspension from iGEM 2010 Distribution Kit of <partinfo>BBa_J13002</partinfo> (Plate 1, Well 13B) and <partinfo>BBa_I13522</partinfo> (Plate 2, Well 8A).

Transformation (1 ul) of <partinfo>BBa_J13002</partinfo>, <partinfo>BBa_I13522</partinfo>, INTEIN, CREAM (Mr.Gene) into 100 ul of TOP10 home-made competent cells.

Transformation of 15-4C5 (1 ul) into in recompetentized E. coli TOP10 carrying <partinfo>BBa_T9002</partinfo>.

Transfomants were plated on LB+Amp 100 ug/ml agar plates, the last one on LB+Amp100+Cm12,5 agar plates and were let grow ON at 37°C.

August, 17th

August, 18th

August, 19th

August, 20th

August, 21st

August, 22nd