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- | <link href="SpryAssets/SpryCollapsiblePanel.css" rel="stylesheet" type="text/css" />
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- | <script src="https://2010.igem.org/User:Shaothing/wiki/SpryCollapsiblePanel.js" type="text/javascript"></script>
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- | <div id="CollapsiblePanel1" class="CollapsiblePanel">
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- | <div class="CollapsiblePanelTab">July 29 (Thu)</div>
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| + | <div class="CollapsiblePanelTab" tabindex="0">July 29 (Thu)</div> |
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| Attendance: Torigata, Takino, Teoh, Yasumoto, Kakuda, Saka, Tamura | | Attendance: Torigata, Takino, Teoh, Yasumoto, Kakuda, Saka, Tamura |
| # Safety lecture for junior members. | | # Safety lecture for junior members. |
- | # Preparation of LB agar plates (26 Amp, 25 Kan, 25 Cam).</div> | + | # Preparation of LB agar plates (26 Amp, 25 Kan, 25 Cam). |
| </div> | | </div> |
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- | <div class="CollapsiblePanelTab">July 31 (Thu)</div>
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- | Attendance: Miyatake, Hirayama, Torigata, Teoh, Tadashi, Yasumoto, Kakuda, Saka
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- | # Meeting
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- | #* Summer project schedule
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- | #* List of genes to clone
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- | </div>
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- | <div class="CollapsiblePanelTab">August 2 (Mon)</div>
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- | Attendance: Torigata, Takino, Teoh, Tadashi, Yasumoto, Saka
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- | # Culture medium preparation
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- | #* LB agar plates (49 antibiotic-less plates)
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- | #* LB liquid medium (500 ml)
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- | # Competent cells preparation - Nojima Method
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- | #* SOB medium (MgCl2 not yet added) -> stored at 4˚C
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- | #* TB buffer -> stored at 4˚C
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- | #* Single-colony streaking of E. coli (strain DH5α) on 2 LB agar plates -> 37˚C incubation o/n
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- | Tomorrow we shall prepare the remainder of the reagents and inoculate SOB with o/n-incubated E. coli.
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- | </div>
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- | <script type="text/javascript">
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| </html> | | </html> |
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- | ===July 29 (Thu)===
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- | Attendance: Torigata, Takino, Teoh, Yasumoto, Kakuda, Saka, Tamura
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- | # Safety lecture for junior members.
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- | # Preparation of LB agar plates (26 Amp, 25 Kan, 25 Cam).
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- |
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- | ===July 31 (Thu)===
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- | Attendance: Miyatake, Hirayama, Torigata, Teoh, Tadashi, Yasumoto, Kakuda, Saka
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- | # Meeting
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- | #* Summer project schedule
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- | #* List of genes to clone
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- |
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- | ===August 2 (Mon)===
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- | Attendance: Torigata, Takino, Teoh, Tadashi, Yasumoto, Saka
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- | # Culture medium preparation
| |
- | #* LB agar plates (49 antibiotic-less plates)
| |
- | #* LB liquid medium (500 ml)
| |
- | # Competent cells preparation - Nojima Method
| |
- | #* SOB medium (MgCl2 not yet added) -> stored at 4˚C
| |
- | #* TB buffer -> stored at 4˚C
| |
- | #* Single-colony streaking of E. coli (strain DH5α) on 2 LB agar plates -> 37˚C incubation o/n
| |
- | Tomorrow we shall prepare the remainder of the reagents and inoculate SOB with o/n-incubated E. coli.
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