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Team:Osaka/notebooktest
From 2010.igem.org
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| - | + | <div class="CollapsiblePanelTab" tabindex="0">July 29 (Thu)</div> | |
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Attendance: Torigata, Takino, Teoh, Yasumoto, Kakuda, Saka, Tamura | Attendance: Torigata, Takino, Teoh, Yasumoto, Kakuda, Saka, Tamura | ||
# Safety lecture for junior members. | # Safety lecture for junior members. | ||
| - | # Preparation of LB agar plates (26 Amp, 25 Kan, 25 Cam).</div> | + | # Preparation of LB agar plates (26 Amp, 25 Kan, 25 Cam).</div> |
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# Meeting | # Meeting | ||
#* Summer project schedule | #* Summer project schedule | ||
| - | #* List of genes to clone | + | #* List of genes to clone</div> |
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| + | <div class="CollapsiblePanelTab">August 2 (Mon)</div> | ||
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Attendance: Torigata, Takino, Teoh, Tadashi, Yasumoto, Saka | Attendance: Torigata, Takino, Teoh, Tadashi, Yasumoto, Saka | ||
# Culture medium preparation | # Culture medium preparation | ||
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#* TB buffer -> stored at 4˚C | #* TB buffer -> stored at 4˚C | ||
#* Single-colony streaking of E. coli (strain DH5α) on 2 LB agar plates -> 37˚C incubation o/n | #* Single-colony streaking of E. coli (strain DH5α) on 2 LB agar plates -> 37˚C incubation o/n | ||
| - | Tomorrow we shall prepare the remainder of the reagents and inoculate SOB with o/n-incubated E. coli. | + | Tomorrow we shall prepare the remainder of the reagents and inoculate SOB with o/n-incubated E. coli.</div> |
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Revision as of 14:42, 15 August 2010
July 29 (Thu)
Attendance: Torigata, Takino, Teoh, Yasumoto, Kakuda, Saka, Tamura
# Safety lecture for junior members.
# Preparation of LB agar plates (26 Amp, 25 Kan, 25 Cam).
July 31 (Thu)
Attendance: Miyatake, Hirayama, Torigata, Teoh, Tadashi, Yasumoto, Kakuda, Saka
# Meeting
#* Summer project schedule
#* List of genes to clone
August 2 (Mon)
Attendance: Torigata, Takino, Teoh, Tadashi, Yasumoto, Saka
# Culture medium preparation
#* LB agar plates (49 antibiotic-less plates)
#* LB liquid medium (500 ml)
# Competent cells preparation - Nojima Method
#* SOB medium (MgCl2 not yet added) -> stored at 4˚C
#* TB buffer -> stored at 4˚C
#* Single-colony streaking of E. coli (strain DH5α) on 2 LB agar plates -> 37˚C incubation o/n
Tomorrow we shall prepare the remainder of the reagents and inoculate SOB with o/n-incubated E. coli.
