Team:Cambridge/PhosphoreumOligos
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EmilyKnott (Talk | contribs) (New page: ==Phosphoreum Oligos== ribA Reverse Primer: Sequence: tactagtagcggccgctgcagAAATGAACGTTAGTTCCAAC Structure: one hairpin in suffix/coding region Tm: 56.27 deg dG: 0.48 kcal/mol luxE ...) |
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ribA Reverse Primer: | ribA Reverse Primer: | ||
- | + | ||
- | + | :Sequence: tactagtagcggccgctgcagAAATGAACGTTAGTTCCAAC | |
- | + | ||
- | + | :Structure: one hairpin in suffix/coding region | |
+ | |||
+ | :Tm: 56.27 deg | ||
+ | |||
+ | :dG: 0.48 kcal/mol | ||
luxE Reverse Primer: | luxE Reverse Primer: | ||
- | + | ||
- | + | :Sequence: tactagtagcggccgctgcagTATGATCTATTTTAAACCTGATGA | |
- | + | ||
- | + | :Structure: one hairpin in suffix | |
+ | |||
+ | :Tm: 56.59 deg | ||
+ | |||
+ | :dG: 0.57 kcal/mol | ||
Pbad Reverse C-end: | Pbad Reverse C-end: | ||
- | + | ||
- | + | :Sequence: tctttatcatacaatctccttaGCTAGCCCAAAAAAAC | |
- | + | ||
- | + | :Structure: one small hairpin in gene | |
+ | |||
+ | :Tm: 53.68 deg | ||
+ | |||
+ | :dG: 2.30 kcal/mol | ||
Lux C forward primer: | Lux C forward primer: | ||
- | |||
- | |||
- | |||
+ | :Sequence: AGGAGATTGTATGATAAAGAAAAT | ||
+ | |||
+ | :Tm: 56.26 | ||
+ | |||
+ | :dG: 1.98 | ||
+ | |||
+ | |||
+ | lux A(XbaI) forward: | ||
+ | |||
+ | :Sequence: TACTGGACActagaGCATCATT | ||
+ | |||
+ | :Tm: 58.46 | ||
+ | |||
+ | :dG: 1.53 | ||
+ | |||
+ | lux A(XbaI) reverse: | ||
+ | |||
+ | :Sequence: AATGATGCtctagTGTCCAGTA | ||
+ | |||
+ | :Tm: 58.46 | ||
+ | |||
+ | :dG: 2.53 | ||
+ | |||
+ | |||
+ | lux E(SpeI) Forward: | ||
+ | |||
+ | :Sequence: GTAGTGGTacCagtGGTGTAAA | ||
+ | |||
+ | :Tm: 58.54 | ||
+ | |||
+ | :dG: 1.41 | ||
+ | |||
+ | lux E(SpeI) reverse: | ||
+ | |||
+ | :Sequence: TTTACACCactGgtACCACTAC | ||
+ | |||
+ | :Tm: 58.54 | ||
+ | |||
+ | :dG: 1.25 | ||
+ | |||
+ | |||
+ | |||
+ | lux G(XbaI) forward: | ||
+ | |||
+ | :Sequence: TGCCTGTCctagaAAACTTT | ||
+ | |||
+ | :Tm: 58.56 | ||
+ | |||
+ | :dG: 1.77 | ||
+ | |||
+ | luxG(XbaI) reverse: | ||
+ | |||
+ | :Sequence: AAAGTTTtctagGACAGGCA | ||
+ | |||
+ | :Tm: 58.56 | ||
- | + | :dG: 1.33 | |
- | |||
- | |||
- | |||
- | |||
- | + | ribB (EcoRI i) forward: | |
- | + | ||
- | + | ||
- | + | ||
+ | :Sequence: GAATATGGTgaGttcAATATGATC | ||
+ | :Tm: 58.39 | ||
- | + | :dG: 1.91 | |
- | + | ribB (EcoRI i) reverse: | |
- | + | ||
- | + | ||
- | + | ||
- | + | :Sequence: GATCATATTgaaCtcACCATATTC | |
- | + | ||
- | + | ||
- | + | ||
+ | :Tm: 58.39 | ||
+ | :dG: 1.91 | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | + | ribB (EcoRI ii) forward: | |
- | + | ||
- | + | ||
- | + | ||
+ | :Sequence: TGAGCCgaaCtcTCAAAC | ||
- | + | :Tm: 59.64 | |
- | + | :dG: 1.26 | |
- | + | ||
- | + | ||
- | dG | + | |
- | reverse | + | ribB (EcoRI ii) reverse: |
- | + | ||
- | + | ||
- | + | ||
- | + | :Sequence: GTTTGAgaGttcGGCTCA | |
- | + | :Tm: 59.64 | |
- | + | ||
- | Tm | + | |
- | + | ||
- | + | :dG: 1.16 | |
- | + | ||
- | + | ||
- | dG | + |
Latest revision as of 16:43, 13 August 2010
Phosphoreum Oligos
ribA Reverse Primer:
- Sequence: tactagtagcggccgctgcagAAATGAACGTTAGTTCCAAC
- Structure: one hairpin in suffix/coding region
- Tm: 56.27 deg
- dG: 0.48 kcal/mol
luxE Reverse Primer:
- Sequence: tactagtagcggccgctgcagTATGATCTATTTTAAACCTGATGA
- Structure: one hairpin in suffix
- Tm: 56.59 deg
- dG: 0.57 kcal/mol
Pbad Reverse C-end:
- Sequence: tctttatcatacaatctccttaGCTAGCCCAAAAAAAC
- Structure: one small hairpin in gene
- Tm: 53.68 deg
- dG: 2.30 kcal/mol
Lux C forward primer:
- Sequence: AGGAGATTGTATGATAAAGAAAAT
- Tm: 56.26
- dG: 1.98
lux A(XbaI) forward:
- Sequence: TACTGGACActagaGCATCATT
- Tm: 58.46
- dG: 1.53
lux A(XbaI) reverse:
- Sequence: AATGATGCtctagTGTCCAGTA
- Tm: 58.46
- dG: 2.53
lux E(SpeI) Forward:
- Sequence: GTAGTGGTacCagtGGTGTAAA
- Tm: 58.54
- dG: 1.41
lux E(SpeI) reverse:
- Sequence: TTTACACCactGgtACCACTAC
- Tm: 58.54
- dG: 1.25
lux G(XbaI) forward:
- Sequence: TGCCTGTCctagaAAACTTT
- Tm: 58.56
- dG: 1.77
luxG(XbaI) reverse:
- Sequence: AAAGTTTtctagGACAGGCA
- Tm: 58.56
- dG: 1.33
ribB (EcoRI i) forward:
- Sequence: GAATATGGTgaGttcAATATGATC
- Tm: 58.39
- dG: 1.91
ribB (EcoRI i) reverse:
- Sequence: GATCATATTgaaCtcACCATATTC
- Tm: 58.39
- dG: 1.91
ribB (EcoRI ii) forward:
- Sequence: TGAGCCgaaCtcTCAAAC
- Tm: 59.64
- dG: 1.26
ribB (EcoRI ii) reverse:
- Sequence: GTTTGAgaGttcGGCTCA
- Tm: 59.64
- dG: 1.16