Team:Cambridge/PhosphoreumOligos

From 2010.igem.org

(Difference between revisions)
(New page: ==Phosphoreum Oligos== ribA Reverse Primer: Sequence: tactagtagcggccgctgcagAAATGAACGTTAGTTCCAAC Structure: one hairpin in suffix/coding region Tm: 56.27 deg dG: 0.48 kcal/mol luxE ...)
 
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ribA Reverse Primer:
ribA Reverse Primer:
-
Sequence: tactagtagcggccgctgcagAAATGAACGTTAGTTCCAAC
+
 
-
Structure: one hairpin in suffix/coding region
+
:Sequence: tactagtagcggccgctgcagAAATGAACGTTAGTTCCAAC
-
Tm: 56.27 deg
+
 
-
dG: 0.48 kcal/mol
+
:Structure: one hairpin in suffix/coding region
 +
 
 +
:Tm: 56.27 deg
 +
 
 +
:dG: 0.48 kcal/mol
luxE Reverse Primer:
luxE Reverse Primer:
-
Sequence: tactagtagcggccgctgcagTATGATCTATTTTAAACCTGATGA
+
 
-
Structure: one hairpin in suffix
+
:Sequence: tactagtagcggccgctgcagTATGATCTATTTTAAACCTGATGA
-
Tm: 56.59 deg
+
 
-
dG: 0.57 kcal/mol
+
:Structure: one hairpin in suffix
 +
 
 +
:Tm: 56.59 deg
 +
 
 +
:dG: 0.57 kcal/mol
Pbad Reverse C-end:
Pbad Reverse C-end:
-
Sequence: tctttatcatacaatctccttaGCTAGCCCAAAAAAAC
+
 
-
Structure: one small hairpin in gene
+
:Sequence: tctttatcatacaatctccttaGCTAGCCCAAAAAAAC
-
Tm: 53.68 deg
+
 
-
dG: 2.30 kcal/mol
+
:Structure: one small hairpin in gene
 +
 
 +
:Tm: 53.68 deg
 +
 
 +
:dG: 2.30 kcal/mol
Lux C forward primer:
Lux C forward primer:
-
Sequence: AGGAGATTGTATGATAAAGAAAAT
 
-
Tm: 56.26
 
-
dG: 1.98
 
 +
:Sequence: AGGAGATTGTATGATAAAGAAAAT
 +
 +
:Tm: 56.26
 +
 +
:dG: 1.98
 +
 +
 +
lux A(XbaI) forward:
 +
 +
:Sequence: TACTGGACActagaGCATCATT
 +
 +
:Tm: 58.46
 +
 +
:dG: 1.53
 +
 +
lux A(XbaI) reverse:
 +
 +
:Sequence: AATGATGCtctagTGTCCAGTA
 +
 +
:Tm: 58.46
 +
 +
:dG: 2.53
 +
 +
 +
lux E(SpeI) Forward:
 +
 +
:Sequence: GTAGTGGTacCagtGGTGTAAA
 +
 +
:Tm: 58.54
 +
 +
:dG: 1.41
 +
 +
lux E(SpeI) reverse:
 +
 +
:Sequence: TTTACACCactGgtACCACTAC
 +
 +
:Tm: 58.54
 +
 +
:dG: 1.25
 +
 +
 +
 +
lux G(XbaI) forward:
 +
 +
:Sequence: TGCCTGTCctagaAAACTTT
 +
 +
:Tm: 58.56
 +
 +
:dG: 1.77
 +
 +
luxG(XbaI) reverse:
 +
 +
:Sequence: AAAGTTTtctagGACAGGCA
 +
 +
:Tm: 58.56
-
lux A(XbaI)
+
:dG: 1.33
-
forward
 
-
TACTGGACActagaGCATCATT
 
-
Tm=58.46
 
-
dG=1.53
 
-
reverse
+
ribB (EcoRI i) forward:
-
AATGATGCtctagTGTCCAGTA
+
-
Tm=58.46
+
-
dG=2.53
+
 +
:Sequence: GAATATGGTgaGttcAATATGATC
 +
:Tm: 58.39
-
lux E(SpeI)
+
:dG: 1.91
-
forward
+
ribB (EcoRI i) reverse:
-
GTAGTGGTacCagtGGTGTAAA
+
-
Tm=58.54
+
-
dG=1.41
+
-
reverse
+
:Sequence: GATCATATTgaaCtcACCATATTC
-
TTTACACCactGgtACCACTAC
+
-
Tm=58.54
+
-
dG=1.25
+
 +
:Tm: 58.39
 +
:dG: 1.91
-
lux G(XbaI)
 
-
forward
 
-
TGCCTGTCctagaAAACTTT
 
-
Tm=58.56
 
-
dG=1.77
 
-
reverse
+
ribB (EcoRI ii) forward:
-
AAAGTTTtctagGACAGGCA
+
-
Tm=58.56
+
-
dG=1.33
+
 +
:Sequence: TGAGCCgaaCtcTCAAAC
-
ribB (EcoRI i)
+
:Tm: 59.64
-
forward
+
:dG: 1.26
-
GAATATGGTgaGttcAATATGATC
+
-
Tm=58.39
+
-
dG=1.91
+
-
reverse
+
ribB (EcoRI ii) reverse:
-
GATCATATTgaaCtcACCATATTC
+
-
Tm=58.39
+
-
dG=1.91
+
-
ribB (EcoRI ii)
+
:Sequence: GTTTGAgaGttcGGCTCA
-
forward
+
:Tm: 59.64
-
TGAGCCgaaCtcTCAAAC
+
-
Tm=59.64
+
-
dG=1.26
+
-
reverse
+
:dG: 1.16
-
GTTTGAgaGttcGGCTCA
+
-
Tm=59.64
+
-
dG=1.16
+

Latest revision as of 16:43, 13 August 2010

Phosphoreum Oligos

ribA Reverse Primer:

Sequence: tactagtagcggccgctgcagAAATGAACGTTAGTTCCAAC
Structure: one hairpin in suffix/coding region
Tm: 56.27 deg
dG: 0.48 kcal/mol

luxE Reverse Primer:

Sequence: tactagtagcggccgctgcagTATGATCTATTTTAAACCTGATGA
Structure: one hairpin in suffix
Tm: 56.59 deg
dG: 0.57 kcal/mol

Pbad Reverse C-end:

Sequence: tctttatcatacaatctccttaGCTAGCCCAAAAAAAC
Structure: one small hairpin in gene
Tm: 53.68 deg
dG: 2.30 kcal/mol

Lux C forward primer:

Sequence: AGGAGATTGTATGATAAAGAAAAT
Tm: 56.26
dG: 1.98


lux A(XbaI) forward:

Sequence: TACTGGACActagaGCATCATT
Tm: 58.46
dG: 1.53

lux A(XbaI) reverse:

Sequence: AATGATGCtctagTGTCCAGTA
Tm: 58.46
dG: 2.53


lux E(SpeI) Forward:

Sequence: GTAGTGGTacCagtGGTGTAAA
Tm: 58.54
dG: 1.41

lux E(SpeI) reverse:

Sequence: TTTACACCactGgtACCACTAC
Tm: 58.54
dG: 1.25


lux G(XbaI) forward:

Sequence: TGCCTGTCctagaAAACTTT
Tm: 58.56
dG: 1.77

luxG(XbaI) reverse:

Sequence: AAAGTTTtctagGACAGGCA
Tm: 58.56
dG: 1.33


ribB (EcoRI i) forward:

Sequence: GAATATGGTgaGttcAATATGATC
Tm: 58.39
dG: 1.91

ribB (EcoRI i) reverse:

Sequence: GATCATATTgaaCtcACCATATTC
Tm: 58.39
dG: 1.91


ribB (EcoRI ii) forward:

Sequence: TGAGCCgaaCtcTCAAAC
Tm: 59.64
dG: 1.26

ribB (EcoRI ii) reverse:

Sequence: GTTTGAgaGttcGGCTCA
Tm: 59.64
dG: 1.16