Team:Michigan/Virus Surface Display

From 2010.igem.org

(Difference between revisions)

Revision as of 21:51, 12 August 2010

7/10/2010 13:00

• digest I719015 (T7 GFPmut3b) and I719005(T7 Promoter) with EcoR1 and Pst1.

7/13/2010 9:00

• inoculate competent cell culture

• harvest I719015, K145001, K117008, K117002, K103006 from registry

7/15/2010 4:30

• streak plate INP NC sample

7/18/2010 5:00

• miniprep INP NC sample

7/21/2010 6:00

• digest INP NC with EcoRI and SpeI

7/23/2010 9:00

• gel electrophoresis of all biobricks

lane 1 - ladder

lane 2 - INP NC sample 1 (K265008)

lane 3 - INP NC sample 2 (K265008)

lane 4 - T7 GFPmut3b (I719015)

lane 5 - T7 Promoter (I719005)

lane 6 - T7 RNA polymerase (K145001)

lane 7 - pLsrA-YFP (K117008)

lane 8 - LsrA promoter (K117002)

lane 9 - OmpA (K103006)

7/25/2010 5:00

• aliquot INP samples and primers for sequencing

7/27/2010 9:00

• miniprep

7/28/2010 4:00

• digest

• gel electrophoresis

Lane 1 (far right): invitrogen 1 kb plus ladder

Lane 2: GFP cut with XbaI and PstI

Lane 3: GFP cut with EcoRI and XbaI

Lane 4: uncut GFP plamid

Lane 5:INP cut with EcoRI and SpeI

Lane 6:INP cut with SpeI and PstI

Lane 7:uncut INP plasmid

Lane 8:Linker cut with XbaI and SpeI

Lane 9:Linker cut with SpeI and PstI

Lane 10: uncut Linker plasmid

Lane 11: OmpA cut with EcoRI and SpeI

Lane 12: OmpA cut with SpeI and PstI

Lane 13:uncut OmpA plasmid

7/30/2010 10:00

• ligation transformation

7/31/2010 9:00

• inoculate minipreps from the INP-linker

@@ Line 1: / Line 1: @@
 {{Michigan Header}}
+{|id:"pili_expression"
+|-valign="top"
+|width="700px" style="padding: 0 20px 0 0; background-color:rgb(190,210,250);"|
 /10/2010
@@ Line 119: / Line 122: @@
 • inoculate minipreps from the INP-linker
+|width="250px" style="background-color:rgb(190,210,250);"|
+===Lab impressions===
+[[Image:Virus01.jpg|middle|250px]]
+[[Image:Virus02.jpg|middle|250px]]
+[[Image:Virus03.jpg|middle|250px]]
+[[Image:Virus04.jpg|middle|250px]]
+[[Image:Virus05.jpg|middle|250px]]
+|}