Team:DTU-Denmark

From 2010.igem.org

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The goal of our project is to enable colonies of E. coli bacteria to transition between production of two different reporter proteins. In our system, switching between states will be induced by exposing the bacteria to light. Each of the states will have a specific frequency associated with it. Such biological “switch” has many potential applications, e.g. easy control of production of additives in industrial biotechnological processes.
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[[Image:DTU_paris.jpg|400px|thumb|right|DTU Team in Paris for the iGEM workshop (Malte is missing)]]
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:The goal of our project is to enable colonies of E. coli bacteria to transition between production of two different reporter proteins. In our system, switching between states will be induced by exposing the bacteria to light. Each of the states will have a specific frequency associated with it. Such biological “switch” has many potential applications, e.g. easy control of production of additives in industrial biotechnological processes.<br><br>Production of first reporter protein will be induced by red light (660 nm). At the same time, production of the other reporter will be suppressed by a coexpressed repressor. Conversely, production of the second reporter can be induced by blue light (470 nm). Bistability of the system is achieved by using two repressors which negatively regulate each other’s expression. This enables the system to sustain state without continuous input, i. e. once production of a reporter protein is initiated, it will persist until the system is forced into the other state. As a proof of concept, we’re using fluorescent proteins as reporter genes which makes it easy to observe and characterise the system. In principle, however, any reporter gene can be  used.
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Production of first reporter protein will be induced by red light (660 nm). At the same time, production of the other reporter will be suppressed by a coexpressed repressor. Conversely, production of the second reporter can be induced by blue light (470 nm). Bistability of the system is achieved by using two repressors which negatively regulate each other’s expression. This enables the system to sustain state without continuous input, i. e. once production of a reporter protein is initiated, it will persist until the system is forced into the other state. As a proof of concept, we’re using fluorescent proteins as reporter genes which makes it easy to observe and characterise the system. In principle, however, any reporter gene can be  used.
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<!--- The Mission, Experiments --->
<!--- The Mission, Experiments --->

Revision as of 09:16, 11 August 2010

DTU-Denmark logo.png
Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety
DTU Team in Paris for the iGEM workshop (Malte is missing)
The goal of our project is to enable colonies of E. coli bacteria to transition between production of two different reporter proteins. In our system, switching between states will be induced by exposing the bacteria to light. Each of the states will have a specific frequency associated with it. Such biological “switch” has many potential applications, e.g. easy control of production of additives in industrial biotechnological processes.

Production of first reporter protein will be induced by red light (660 nm). At the same time, production of the other reporter will be suppressed by a coexpressed repressor. Conversely, production of the second reporter can be induced by blue light (470 nm). Bistability of the system is achieved by using two repressors which negatively regulate each other’s expression. This enables the system to sustain state without continuous input, i. e. once production of a reporter protein is initiated, it will persist until the system is forced into the other state. As a proof of concept, we’re using fluorescent proteins as reporter genes which makes it easy to observe and characterise the system. In principle, however, any reporter gene can be used.


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