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Team:Michigan/Protocols
From 2010.igem.org
(Difference between revisions)
| Line 51: | Line 51: | ||
[[Media:8-8-2010_Protocol_for_gel_extraction.pdf|Gel extraction]] | [[Media:8-8-2010_Protocol_for_gel_extraction.pdf|Gel extraction]] | ||
| - | [[Media:Ligation_Protocol.pdf|Ligation]] | + | [[Media:8-8-2010_Protocol_for_gel_extraction.pdf|Updated T4 DNA Ligase Protocol (Not quick ligase)]] |
| - | *Ginko Bioworks Protocol | + | *[[Media:Ligation_Protocol.pdf|Ligation]] |
| - | *Jeremy Minty Protocol | + | **Ginko Bioworks Protocol |
| + | **Jeremy Minty Protocol | ||
===Group-Specific Protocols=== | ===Group-Specific Protocols=== | ||
====Oil Sands==== | ====Oil Sands==== | ||
Revision as of 02:35, 11 August 2010
Protocols
Using Lab Equipment
Obtaining Deionized Water in the ERB
Enigeering Research Building Autoclave
Epifluorescence Microscope Usage - H.H. Dow
Cell Culture
Making cultures from a -80C freezer stock
P. putida KT2440 antibotic resistance tolerance
DNA Manipulation
- Getting parts from the 360 well registry plates
- Competent cell preparation
- Heat shock
Transformation-electroporation
- Getting parts from the 360 well registry plates
- Competent cell preparation
- Electroporation
Updated T4 DNA Ligase Protocol (Not quick ligase)
- Ligation
- Ginko Bioworks Protocol
- Jeremy Minty Protocol
Group-Specific Protocols
Oil Sands
Revised biofilm assay protocol
Quorum Sensing
General Outline for Biobrick Manipulations
