Team:Cambridge/Oligos

From 2010.igem.org

(Difference between revisions)
(→Notes on alterations to the SLOCK pHK724 clone)
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*A good [http://www.bio.cmu.edu/courses/03441/TermPapers/97TermPapers/lux/operon.html summary] of the lux operon with quality references
*A good [http://www.bio.cmu.edu/courses/03441/TermPapers/97TermPapers/lux/operon.html summary] of the lux operon with quality references
*How to design a [http://partsregistry.org/Ribosome_Binding_Sites/Design RBS]
*How to design a [http://partsregistry.org/Ribosome_Binding_Sites/Design RBS]
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=lux oligos=
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==luxI ==      
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===forward===
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gaattcgcggccgcttctagagAAGGGAGGTTGGTATGACTAT
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dG=0.11
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Tm=58.45
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===reverse coding===
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atgatcatcgccggcgacgtcTTAATTTAAGACTGCTTTTTTAAACT
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dG=-0.34
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Tm=57.3
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===reverse RBS===
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atgatcatcgccggcgacgtcATAATATACTTAGTATTTAAAATAAATTAATGATTTA
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dG=-0.34
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Tm=56.83
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==luxC==
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===forward===      
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gaattcgcggccgcttctagagAGGGAAAATAATGAATAAATGTAT
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Tm=56.07
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dG=0.76
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===reverse coding===
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atgatcatcgccggcgacgtcCTATGGGACAAAAACTAAAAAC
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dG=-0.77
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Tm=56.58
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===reverse RBS===
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atgatcatcgccggcgacgtcTAACTATGGGACAAAAACTAAA
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dG=-0.34
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Tm=55.31
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==luxD==
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===forward===
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gaattcgcggccgcttctagagAAGGAAATTATATGAAAGATGAAA
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Tm=57.08
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dG=0.11
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===reverse coding===
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atgatcatcgccggcgacgtcTTAAGCCAATTCTAATAATTCATT
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dG=-0.34
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Tm=57.19
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===reverse RBS===
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atgatcatcgccggcgacgtcTTGGTGATTCTGTTTATTTAAG
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dG= -0.34
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Tm=55.69
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==luxA==
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===forward===
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gaattcgcggccgcttctagagAAAGGAATAGAGTATGAAGTTTGG
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Tm=58.8
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dG=0.76
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===reverse coding===
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atgatcatcgccggcgacgtcTTATTTAGGTTCTTTTAAGAAAGGAG
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dG=-0.34
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Tm=58.66
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===reverse RBS===
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atgatcatcgccggcgacgtcTTATCTCTAGTATCAAATAAGTAATTTATTTAGG
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dG=-0.34
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Tm=58.84
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==luxB==
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===forward===
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gaattcgcggccgcttctagagTAAGGAACAAGTTATGAAATTTG
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Tm=57.09
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dG=0.47
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===reverse coding===
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atgatcatcgccggcgacgtcTTATGGTAAATTCATTTCGATT
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dG=-0.34
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Tm=57.2
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===reverse RBS===
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atgatcatcgccggcgacgtcAATACCTAATATCAACAGAAATTTAAA
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dG=-0.34
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Tm=56.89
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==luxE==
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===forward===
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gaattcgcggccgcttctagagACTGGAGAGGGTATGACTGT
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Tm=58.15
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dG=0.76
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===reverse coding===
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atgatcatcgccggcgacgtcTTAATCCTTGATATTCTTTTGTATG
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dG=-0.34
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Tm=57.5
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===reverse RBS===
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atgatcatcgccggcgacgtcAACTTAATCCTTGATATTCTTTTGTAT
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dG=-0.34
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Tm=57.96
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==luxG==
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===forward===
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gaattcgcggccgcttctagagAAGGATTAAGTTATGATTGTTGA
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Tm=56.52
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dG=0.11
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===reverse coding===
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atgatcatcgccggcgacgtcTTATACGTATGCAAAAGCATC
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dG=-1.21
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Tm=57.48
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===reverse RBS===
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atgatcatcgccggcgacgtcAAAGCCGGATTAATAATCTG
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dG=-3.57
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Tm=57.82
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alternative ===reverse RBS===
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atgatcatcgccggcgacgtcTTTATATTCTCTTTATACGTATGCAA
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dG=-0.34
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Tm=57.36
===luxICDABE operon===
===luxICDABE operon===

Revision as of 11:33, 10 August 2010

Retrieved from "http://2010.igem.org/Team:Cambridge/Oligos"