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Team:Newcastle/9 August 2010
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RachelBoyd (Talk | contribs) (New page: =Transformation= ==Results/Aims== *pVeg transformed sucessfully- From this we will set up an overnight broth culture for plasmid extraction *''lacI''- To show that we have sucessfully t...) |
RachelBoyd (Talk | contribs) (→ligation) |
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*''lacI''- To show that we have sucessfully transformed form ''lacI'' we will select 12 colonies and plate them on a section of an agar plate as well as setting up an overnight broth culture. | *''lacI''- To show that we have sucessfully transformed form ''lacI'' we will select 12 colonies and plate them on a section of an agar plate as well as setting up an overnight broth culture. | ||
| - | = | + | =Ligation= |
==Aims== | ==Aims== | ||
Today we will transform some competent ''E.coli'' DH5alpha with the ligation products: 1:5,1:3 and the vector | Today we will transform some competent ''E.coli'' DH5alpha with the ligation products: 1:5,1:3 and the vector | ||
Revision as of 09:10, 9 August 2010
Contents |
Transformation
Results/Aims
- pVeg transformed sucessfully- From this we will set up an overnight broth culture for plasmid extraction
- lacI- To show that we have sucessfully transformed form lacI we will select 12 colonies and plate them on a section of an agar plate as well as setting up an overnight broth culture.
Ligation
Aims
Today we will transform some competent E.coli DH5alpha with the ligation products: 1:5,1:3 and the vector
