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Team:Cambridge/Bioluminescence/Bacterial Luciferases
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*<i>luxA</i> and <i>luxB</i> form the luciferase part of the system, they emit light using the substrate <b>tetradecanal</b> | *<i>luxA</i> and <i>luxB</i> form the luciferase part of the system, they emit light using the substrate <b>tetradecanal</b> | ||
*<i>luxC</i>, <i>luxD</i> and <i>luxE</i> are involved in the biosynthesis of tetradecanal from readily available substrates | *<i>luxC</i>, <i>luxD</i> and <i>luxE</i> are involved in the biosynthesis of tetradecanal from readily available substrates | ||
| - | == | + | ==Bioluminescent species== |
The Cambridge team is looking into these bacterial luciferases: | The Cambridge team is looking into these bacterial luciferases: | ||
* <i>Vibrio fischeri</i> forms symbioses with squid to prevent the squid from casting a shadow on moonlit nights. Due to this their lux proteins are non-functional above 30 degrees | * <i>Vibrio fischeri</i> forms symbioses with squid to prevent the squid from casting a shadow on moonlit nights. Due to this their lux proteins are non-functional above 30 degrees | ||
Revision as of 00:45, 9 August 2010
Bioluminescence: Bacterial Luciferases
Pathway
Bacterial lux operons emit light using five enzymes:
- luxA and luxB form the luciferase part of the system, they emit light using the substrate tetradecanal
- luxC, luxD and luxE are involved in the biosynthesis of tetradecanal from readily available substrates
Bioluminescent species
The Cambridge team is looking into these bacterial luciferases:
- Vibrio fischeri forms symbioses with squid to prevent the squid from casting a shadow on moonlit nights. Due to this their lux proteins are non-functional above 30 degrees
- Photorhabdus luminescens lives in the gut of nematodes. The purpose of its bioluminescence is not understood but its lux casette is widely used in reporters
- Vibrio (formerly Photobacterium) phosophoreum emits a very blue light, perhaps partly through its use of [http://partsregistry.org/Part:BBa_K216007 lumazine]
