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Team:EPF Lausanne/Labbook protocols
From 2010.igem.org
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On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much. | On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much. | ||
| - | *[https://static.igem.org/mediawiki/2010/f/f9/ | + | *[https://static.igem.org/mediawiki/2010/archive/f/f9/20100727133405%21Prot_adapt_pH.pdf Adapt pH] |
*[https://static.igem.org/mediawiki/2010/6/6f/Prot_agarose_gel.pdf Agarose Gel] | *[https://static.igem.org/mediawiki/2010/6/6f/Prot_agarose_gel.pdf Agarose Gel] | ||
*[https://static.igem.org/mediawiki/2010/6/63/Prot_agar_plates.pdf Agar plates production] | *[https://static.igem.org/mediawiki/2010/6/63/Prot_agar_plates.pdf Agar plates production] | ||
| - | *[https://static.igem.org/mediawiki/2010/f/f9/Prot_autoclave.pdf Autoclave ] | + | *[https://static.igem.org/mediawiki/2010/f/f9/Prot_autoclave.pdf Autoclave] |
| - | * | + | *[https://static.igem.org/mediawiki/2010/6/6b/Prot_asaia_competence.pdf Competent Asaia] |
*[https://static.igem.org/mediawiki/2010/8/82/Prot_electrophoresis.pdf Electrophoresis] | *[https://static.igem.org/mediawiki/2010/8/82/Prot_electrophoresis.pdf Electrophoresis] | ||
| - | * Growing Asaia | + | *[https://static.igem.org/mediawiki/2010/8/82/Prot_growing_asaia.pdf Growing Asaia ] |
*[https://static.igem.org/mediawiki/2010/6/6a/Prot_ligation.pdf Ligation] | *[https://static.igem.org/mediawiki/2010/6/6a/Prot_ligation.pdf Ligation] | ||
*[https://static.igem.org/mediawiki/2010/f/f1/Prot_nanodrop.pdf Measuring DNA concentration using NanoDrop] | *[https://static.igem.org/mediawiki/2010/f/f1/Prot_nanodrop.pdf Measuring DNA concentration using NanoDrop] | ||
| - | * Miniprep --> use Miniprep Kit | + | * Miniprep --> use Miniprep Kit (Protocol included in kit) |
*[https://static.igem.org/mediawiki/2010/e/ec/Prot_OD.pdf Optical Density (OD)] | *[https://static.igem.org/mediawiki/2010/e/ec/Prot_OD.pdf Optical Density (OD)] | ||
| - | *[https://static.igem.org/mediawiki/2010/6/ | + | *[https://static.igem.org/mediawiki/2010/5/54/Protocol_pcr_roche.pdf PCR (using the Roche Expand High Fidelity PCR System dNTPack ] |
| - | * Purification --> use Purification Kit | + | *[https://static.igem.org/mediawiki/2010/6/6a/Prot_pcr_iproof.pdf PCR (using iProof BIORAD)] |
| + | * Purification --> use Purification Kit (Protocol included in kit) | ||
*[https://static.igem.org/mediawiki/2010/1/1e/Prot_transformation.pdf Transformation of competent cells] | *[https://static.igem.org/mediawiki/2010/1/1e/Prot_transformation.pdf Transformation of competent cells] | ||
*[https://static.igem.org/mediawiki/2010/2/2f/Prot_UV_imaging.pdf UV Imaging] | *[https://static.igem.org/mediawiki/2010/2/2f/Prot_UV_imaging.pdf UV Imaging] | ||
| - | *Using the plate reader (Solange) | + | *Using the plate reader (Solange or Stefano) |
*[https://static.igem.org/mediawiki/2010/a/a2/Prot_digestion.pdf Digestion] | *[https://static.igem.org/mediawiki/2010/a/a2/Prot_digestion.pdf Digestion] | ||
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= Media Recipes = | = Media Recipes = | ||
Latest revision as of 19:36, 8 August 2010
Lab Protocols
On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much.
- Adapt pH
- Agarose Gel
- Agar plates production
- Autoclave
- Competent Asaia
- Electrophoresis
- Growing Asaia
- Ligation
- Measuring DNA concentration using NanoDrop
- Miniprep --> use Miniprep Kit (Protocol included in kit)
- Optical Density (OD)
- PCR (using the Roche Expand High Fidelity PCR System dNTPack
- PCR (using iProof BIORAD)
- Purification --> use Purification Kit (Protocol included in kit)
- Transformation of competent cells
- UV Imaging
- Using the plate reader (Solange or Stefano)
- Digestion
Media Recipes
Syntax
Convention if you want to upload a new protocol. When uploading a file it please rename it in the following way:
EPFL_prot_yourProcedureName.yourExtension
