Team:ETHZ Basel/Biology/Status
From 2010.igem.org
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= testing of biobricks = | = testing of biobricks = | ||
+ | == testing of che-fusions == | ||
+ | We're currently trying to establish an assay to test for the chemotactic behavour of ''E. coli''. A first try with a "syringe test" failed. We'll redo the test and are looking for other possibilites. Maybe a swarm test? | ||
+ | |||
+ | |||
+ | |||
+ | == testing of localicer-fusions == | ||
+ | |||
+ | We are planning to use a fluorescence microscope in order to visualize the distribution of localizer-cyFP fusions within a single ''E. coli''. | ||
+ | |||
+ | == testing of couplers == | ||
+ | We're planning to use the setup as for the testing of the localizers. In this case we're going to use the following biobricks: | ||
+ | *localizer-PhyB and PIF3-cyFP or | ||
+ | *localizer-PIF3 and PhyB-cyFP | ||
+ | Images should look as in testing of localizer-fusions. Preliminary clarificaiton of the suitability of the approach are to be done. | ||
= bringing ''E. lemming'' alive = | = bringing ''E. lemming'' alive = |
Revision as of 15:05, 6 August 2010
generation of subparts
Protein | primers | PCR product | library of transformants | single colonies | storage vector | insert | |
---|---|---|---|---|---|---|---|
che proteins | CheY-A | recieved | purified on stock | on agar plate | not available | purified on stock | on stock |
CheY-C | recieved | purified in stock | on agar plate | not available | purified on stock | on stock | |
CheB-A | recieved | purified in stock | on agar plate | 3 on agar plate | purified on stock | on stock | |
CheB-C | recieved | purified in stock | on agar plate | 3 on agar plate | purified on stock | on stock | |
CheR-A | recieved | purified in stock | on agar plate | 3 on agar plate | digest has to be redone | ||
CheR-C | recieved | purified in stock | on agar plate | 3 on agar plate | digest has to be redone | ||
localizers | TrigF-A | recieved | purified in stock | on agar plate | 3 on agar plate | purified on stock | |
MreB-C | recieved | purified in stock | in process | ||||
tetR-A | recieved | purified in stock | on agar plate | 3 on agar plate | purified on stock | on stock | |
couplers | PIF3-A | recieved | purified in stock | on agar plate | 3 on agar plate | purified on stock | |
PIF3-C | recieved | purified in stock | on agar plate | 3 on agar plate | purified on stock | ||
PhyB-C | recieved | purified in stock | on agar plate | 3 on agar plate | purified on stock | ||
reporters | cyFP-A | recieved | purified in stock | on agar plate | 3 on agar plate | purified on stock | on stock |
cyFP-C | recieved | purified in stock | on agar plate | 3 on agar plate | purified on stock |
generation of biobricks
testing of biobricks
testing of che-fusions
We're currently trying to establish an assay to test for the chemotactic behavour of E. coli. A first try with a "syringe test" failed. We'll redo the test and are looking for other possibilites. Maybe a swarm test?
testing of localicer-fusions
We are planning to use a fluorescence microscope in order to visualize the distribution of localizer-cyFP fusions within a single E. coli.
testing of couplers
We're planning to use the setup as for the testing of the localizers. In this case we're going to use the following biobricks:
- localizer-PhyB and PIF3-cyFP or
- localizer-PIF3 and PhyB-cyFP
Images should look as in testing of localizer-fusions. Preliminary clarificaiton of the suitability of the approach are to be done.