Team:ETHZ Basel/Biology/Status

From 2010.igem.org

(Difference between revisions)
Line 1: Line 1:
{{ETHZ_Basel10}}
{{ETHZ_Basel10}}
 +
= generation of subparts =
{| border="1"
{| border="1"
|+ subparts
|+ subparts
-
! Protein !! terminus on fusion !! status
+
! !! Protein !! primers !! PCR product !! library of transformants !! single colonies !! storage vector !! insert
|-
|-
-
! CheY
+
! che proteins
-
| N || sequencing  
+
| CheY-A || recieved || sequencing || || || ||
|-
|-
-
! CheY
+
!  
-
| C || sequencing
+
| CheY-C || recieved || || || || ||
|-
|-
-
! CheR
+
!  
-
| N || sequencing
+
| CheB-A || recieved || || || || ||
|-
|-
-
! CheR
+
!  
-
| C || sequencing
+
| CheB-C || recieved || || || || ||
|-
|-
-
! CheB
+
!  
-
| N || sequencing
+
| CheR-A || recieved || || || || ||
|-
|-
-
! CheB
+
!  
-
| C || sequencing
+
| CheR-C || recieved || || || || ||
|-
|-
-
! TrigF
+
! localizers
-
| N (binds N-terminal to ribosome) || PCR
+
| TrigF-A || recieved || || || || ||
|-
|-
-
! MreB
+
!  
-
| N || ligation
+
| MreB-C || recieved || || || || ||
|-
|-
-
! MreB
+
!  
-
| C || ligation
+
| tetR-A || recieved || || || || ||
|-
|-
-
! tetR
+
! couplers
-
| N || ligation
+
| PIF3-A || recieved || || || || ||
|-
|-
-
! tetR
+
!  
-
| C || ligation
+
| PIF3-C || recieved || || || || ||
|-
|-
-
! PhyB
+
!  
-
| N || ligation
+
| PhyB-C || recieved || || || || ||
|-
|-
-
! PhyB
+
! reporters
-
| C || ligation
+
| cyFP-A || recieved || || || || ||
|-
|-
-
! PhyB
+
!  
-
| N || ligation
+
| cyFP-C || recieved || || || || ||
-
|-
+
|-  
-
! PhyB
+
-
| C || ligation
+
-
|-
+
-
! PIF3
+
-
| N || ligation
+
-
|-
+
-
! PIF3
+
-
| C || ligation
+
-
|-
+
-
! YFP
+
-
| N ||  
+
-
|-
+
-
! YFP
+
-
| C ||
+
-
|-
+
-
! GFP
+
-
| N ||
+
-
|-
+
-
! GFP
+
-
| C ||
+
|}
|}

Revision as of 14:34, 6 August 2010

generation of subparts

subparts
Protein primers PCR product library of transformants single colonies storage vector insert
che proteins CheY-A recieved sequencing
CheY-C recieved
CheB-A recieved
CheB-C recieved
CheR-A recieved
CheR-C recieved
localizers TrigF-A recieved
MreB-C recieved
tetR-A recieved
couplers PIF3-A recieved
PIF3-C recieved
PhyB-C recieved
reporters cyFP-A recieved
cyFP-C recieved


timetable

experimental plan
Retrieved from "http://2010.igem.org/Team:ETHZ_Basel/Biology/Status"