Revision as of 13:08, 6 August 2010

Lab Book

On LB agar plates:

TOP10
MG1655
W3110 hns 93-1
BW25113

On LB agar + kan plates:

BW25113 Δhns::kan
BW25113 ΔtraA::kan

Incubated at 37°C overnight

all strains grew with individual colonies.

Inoculated single bacterial colony (ATP10) in 12ml LB, incubated at 37°C with shaking (180 rpm) overnight.

Inoculated 400ml LB with 5ml TOP10 overnight culture, incubate at 37°C with shaking (180rpm) put on at 11:40am

OD₆₀₀ measurements:

Time	OD₆₀₀ (1)	OD₆₀₀ (2)
12:20	-0.026	-0.032
2:30	0.437	0.530

@@ Line 1: / Line 1: @@
 =Lab Book=
 ==27.07.10==
-===Streaking out of bacterial cultures (Peter & Anja)===
+===1. Streaking out of bacterial cultures (Peter & Anja)===
 On LB agar plates:
-TOP10
+ TOP10
-MG1655
+ MG1655
-W3110 hns 93-1
+ W3110 hns 93-1
-BW25113
+ BW25113
 On LB agar + kan plates:
-BW25113 Δhns::kan
+ BW25113 Δhns::kan
-BW25113 ΔtraA::kan
+ BW25113 ΔtraA::kan
+Incubated at 37°C overnight
+==28.07.10==
+===E1.Streaking out of bacterial cultures [cont] (Peter & Anja)===
+all strains grew with individual colonies.
+===E2.Set up overnight culture of TOP10 (Emily & Anja)===
+Inoculated single bacterial colony (ATP10) in 12ml LB, incubated at 37°C with shaking (180 rpm) overnight.
+==29.07.10==
+Inoculated 400ml LB with 5ml TOP10 overnight culture, incubate at 37°C with shaking (180rpm) put on at 11:40am
+OD<sub>600</sub> measurements:
+{| class="wikitable"
+|-
+! Time
+! OD<sub>600</sub> (1)
+! OD<sub>600</sub> (2)
+|-
+| 12:20
+| -0.026
+| -0.032
+|-
+| 2:30
+| 0.437
+| 0.530
+|}