Team:Cambridge/Bioluminescence/Photinus pyralis

We are investigating a number of firefly luciferases. As a comparison for our future experiments we will begin by cloning the existing Biobrick for [http://partsregistry.org/Part:BBa_I712019 wild type P. pyralis luciferase] into our strains of interest.

Our project will focus on characterising both this existing luciferase and those we hope to submit to the registry:

• An enhanced P. pyralis luciferase with increased brightness
• A number of differently coloured versions of L. cruciata luciferase

We intend to synthesise these luciferases on operons also containing the LRE for the firefly species in question.