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Team:Cambridge/Bioluminescence/Photinus pyralis
From 2010.igem.org
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| - | We are investigating a number of firefly luciferases. As a comparison for our future experiments we will begin by cloning the existing Biobrick for | + | We are investigating a number of firefly luciferases. As a comparison for our future experiments we will begin by cloning the existing Biobrick for [http://partsregistry.org/Part:BBa_I712019 wild type <i>P. pyralis</i> luciferase] into our strains of interest. |
Our project will focus on characterising both this existing luciferase and those we hope to submit to the registry: | Our project will focus on characterising both this existing luciferase and those we hope to submit to the registry: | ||
Revision as of 19:57, 3 August 2010
Bioluminescence: Firefly Luciferases
We are investigating a number of firefly luciferases. As a comparison for our future experiments we will begin by cloning the existing Biobrick for [http://partsregistry.org/Part:BBa_I712019 wild type P. pyralis luciferase] into our strains of interest.
Our project will focus on characterising both this existing luciferase and those we hope to submit to the registry:
- An enhanced P. pyralis luciferase with increased brightness
- A number of differently coloured versions of L. cruciata luciferase
We intend to synthesise these luciferases on operons also containing the LRE for the firefly species in question.
