"
Team:Harvard/fences/parts
From 2010.igem.org
(Difference between revisions)
| Line 46: | Line 46: | ||
<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr> | <tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr> | ||
<tr><td>Arp2mutForward</td><td>Point mutagenesis to remove restriction enzyme site</td><td>5'-CAA GAA GCT TGA CTT GTC TAC GAA CTC TTC CAT TTC-3'</td></tr> | <tr><td>Arp2mutForward</td><td>Point mutagenesis to remove restriction enzyme site</td><td>5'-CAA GAA GCT TGA CTT GTC TAC GAA CTC TTC CAT TTC-3'</td></tr> | ||
| - | <tr><td> | + | <tr><td>Arp2mutReverse</td><td>Point mutagenesis to remove restriction enzyme site</td><td>5'-GAA ATG GAA GAA CTC GTA GAC AAG TCA AGC TCC TTG-3'</td></tr> |
<tr><td>VP5</td><td>HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert</td><td></td></tr> | <tr><td>VP5</td><td>HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert</td><td></td></tr> | ||
<tr><td>VP6</td><td>gusA-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr> | <tr><td>VP6</td><td>gusA-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr> | ||
Revision as of 17:56, 3 August 2010
parts and primers
Vectors
| Name | Original Name | Description | Resistance | Resistance (Plants) | Sequence |
| V1 | TEXT | INSERT TEXT HERE | Kanamycin | pat | |
| V2 | TEXT | INSERT TEXT HERE | Kanamycin | nptII | |
| V3 | TEXT | INSERT TEXT HERE | Kanamycin | pat | |
| V4 | TEXT | INSERT TEXT HERE | Kanamycin | nptII | |
| V5 | TEXT | INSERT TEXT HERE | Kanamycin | nptII |
Primers
| Name | Description | Sequence |
| Arp2mutForward | Point mutagenesis to remove restriction enzyme site | 5'-CAA GAA GCT TGA CTT GTC TAC GAA CTC TTC CAT TTC-3' |
| Arp2mutReverse | Point mutagenesis to remove restriction enzyme site | 5'-GAA ATG GAA GAA CTC GTA GAC AAG TCA AGC TCC TTG-3' |
| VP5 | HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert | |
| VP6 | gusA-NheI reverse primer for Reporter Series 1 insert | |
| VP7 | HindIII-biobrick multiple cloning site-smGFP-forward primer for Reporter Series 1 insert | |
| VP8 | smGFP-NheI reverse primer for Reporter Series 1 insert | |
| VP9 | Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert | |
| VP11 | Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert | |
| VP12 | 1 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | |
| VP13 | 2 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | |
| VP14 | 3 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | |
| VP15 | 4 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | |
| VP16 | 5 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | |
| VP17 | 1 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP18 | 2 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP19 | 3 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP20 | 4 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP21 | 5 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP22 | 6 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP23 | 7 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP24 | 8 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP25 | 9 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP26 | 1 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
| VP27 | 2 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
| VP28 | 3 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
| VP29 | 4 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
| VP30 | 5 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
| VP33 | 1 of 2 for sequencing V7 and V8 biobrick cloning site | |
| VP34 | 2 of 2 for sequencing V7 and V8 biobrick cloning site |
