"
Team:Cambridge/References/ProjectBioluminescence/Experiments
From 2010.igem.org
(Difference between revisions)
m (header) |
m (→3. Luciferin Regenerating Enzyme: spacing) |
||
| Line 26: | Line 26: | ||
</div> | </div> | ||
</html> | </html> | ||
| - | |||
{{:Team:Cambridge/Templates/footerMinimal}} | {{:Team:Cambridge/Templates/footerMinimal}} | ||
Revision as of 10:54, 3 August 2010
Bioluminescence: Experiments
Firefly
1. Test luminescence with the Luciferase from the registry
- Transform WT E.coli with the luciferase from the registry.
- Feed with D-luciferin, develop a method for measuring light output
- Variables - concentration of inducer (if luciferase promoter inducible), concentration of D-luciferin
- Controls - no luciferin, no inducer
2. Test use of CHBT as a substrate
With sucessfully transformed cells from 1 compare emission when adding:
- D-luciferin
- CHBT
- CHBT + D-Cysteine
- CHBT + L-Cysteine
- (CHBT + L-Luciferin? if necessary)
3. Luciferin Regenerating Enzyme
