Team:TU Delft/1 July 2010 content

From 2010.igem.org

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(New page: ===Lab Work=== {| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1" |Sample |medium |day 1 |day 2 |- |no cells |E2 medium without carbon |0.00 |0.00...)
 
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===Lab Work===
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=Lab Work=
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==''Pseudomonas Putida'' GPO1==
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The second day of the grow process of ''Pseudomonas Putida'' GPO1
{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1"
{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1"
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|Sample  
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|'''Sample'''
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|medium
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|'''Medium'''
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|day 1
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|'''day 1 = 30 June'''
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|day 2
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|'''day 2 = 1 July'''
|-
|-
|no cells
|no cells
-
|E2 medium without carbon
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|E2 without carbon
|0.00
|0.00
|0.00
|0.00
|-
|-
|no cells
|no cells
-
|E2 + 1% LB
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|LB
 +
|0.00
|0.00
|0.00
-
|0.01
 
-
|-
 
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|''Pseudomonas Putida'' GPO1 *
 
-
|E2 + 1% LB
 
-
|
 
-
|
 
|-
|-
|no cells
|no cells
|E2 + 1% LB
|E2 + 1% LB
|0.00
|0.00
-
|0.00
+
|0.01
|-
|-
|''Pseudomonas Putida'' GPO1 *
|''Pseudomonas Putida'' GPO1 *
|E2 + 1% LB
|E2 + 1% LB
-
|
+
|0.01
-
|
+
|0.05
|-
|-
|no cells
|no cells
|E2 + 10% LB
|E2 + 10% LB
-
|
+
|0.00
-
|
+
|0.00
|-
|-
|''Pseudomonas Putida'' GPO1 *
|''Pseudomonas Putida'' GPO1 *
|E2 + 10% LB
|E2 + 10% LB
-
|
+
|0.34
-
|
+
|0.19
|-
|-
|no cells
|no cells
|E2 + 1% octane
|E2 + 1% octane
-
|
+
|0.00
-
|
+
|0.00
|-
|-
|''Pseudomonas Putida'' GPO1 *
|''Pseudomonas Putida'' GPO1 *
|E2 + 1% octane
|E2 + 1% octane
-
|
+
|0.00
-
|
+
|0.00
-
|-
+
-
|no cells
+
-
|E2 + 10% octane
+
-
|
+
-
|
+
|-
|-
|''Pseudomonas Putida'' GPO1 *
|''Pseudomonas Putida'' GPO1 *
|E2 + 10% octane
|E2 + 10% octane
-
|
+
|0.29
-
|
+
|0.18
|}
|}
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* average of 3 colonies
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'''*''' average of 3 colonies
 +
 
 +
''Pseudomonas Putida'' GPO1 can grow on 10% octane as carbon source.
 +
 
 +
==Competent cells==
 +
Top10 cells were cultivated for making competent cells.

Latest revision as of 19:21, 2 August 2010

Lab Work

Pseudomonas Putida GPO1

The second day of the grow process of Pseudomonas Putida GPO1

Sample Medium day 1 = 30 June day 2 = 1 July
no cells E2 without carbon 0.00 0.00
no cells LB 0.00 0.00
no cells E2 + 1% LB 0.00 0.01
Pseudomonas Putida GPO1 * E2 + 1% LB 0.01 0.05
no cells E2 + 10% LB 0.00 0.00
Pseudomonas Putida GPO1 * E2 + 10% LB 0.34 0.19
no cells E2 + 1% octane 0.00 0.00
Pseudomonas Putida GPO1 * E2 + 1% octane 0.00 0.00
Pseudomonas Putida GPO1 * E2 + 10% octane 0.29 0.18

* average of 3 colonies

Pseudomonas Putida GPO1 can grow on 10% octane as carbon source.

Competent cells

Top10 cells were cultivated for making competent cells.

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