Team:EPF Lausanne/Notebook asaia
From 2010.igem.org
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== Asaia == | == Asaia == | ||
- | Today we transform competent Asaia with a GFP + kanamycin (resistance) following this [https://static.igem.org/mediawiki/2010/a/ac/EPFL_prot_Electroporation_asaia.pdf protocols]. We'll waiting 2 days to see if it works. | + | Today we transform competent Asaia with a GFP + kanamycin (resistance) following this [https://static.igem.org/mediawiki/2010/a/ac/EPFL_prot_Electroporation_asaia.pdf protocols]. We plate it and we'll waiting 2 days to see if it works. |
+ | |||
+ | we do digestion to create our first biobrick. We will make the ligation and the transformation in E-Coli tomorrow. | ||
== Other stuff == | == Other stuff == |
Revision as of 16:38, 2 August 2010
Contents |
Calendar
Overview
Here is an animation to illustrate our acutal goal.
Organisation
Here is our actual flowchart. For better quality, just click two time on the picture to see it on full size.
02-08-2010
Asaia
Today we transform competent Asaia with a GFP + kanamycin (resistance) following this protocols. We plate it and we'll waiting 2 days to see if it works.
we do digestion to create our first biobrick. We will make the ligation and the transformation in E-Coli tomorrow.
Other stuff
We make some GLY medium with Agar.