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Team:Cambridge/Notebook/Week3
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| - | == Monday== | + | ==Monday== |
===Afternoon=== | ===Afternoon=== | ||
* Theo created a plan for an <html><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K325000">LRE Biobrick</a></html>, and submitted it to DNA 2.0 for a quote. | * Theo created a plan for an <html><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K325000">LRE Biobrick</a></html>, and submitted it to DNA 2.0 for a quote. | ||
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* We met with Dr. Summers to discuss quiescence. He was very encouraging but have yet to settle IP concerns. He also suggested considering mopping up Rcd from a leaky inducible promoter with antisense RNA. | * We met with Dr. Summers to discuss quiescence. He was very encouraging but have yet to settle IP concerns. He also suggested considering mopping up Rcd from a leaky inducible promoter with antisense RNA. | ||
| - | == Wednesday== | + | ==Wednesday== |
Inoculated a broth with Top 10. Making competent cells tomorrow. | Inoculated a broth with Top 10. Making competent cells tomorrow. | ||
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To do: prepare competent cells | To do: prepare competent cells | ||
| - | == Friday== | + | ==Friday== |
* Preparing order for sythesis | * Preparing order for sythesis | ||
* Theo aligned 300 luciferase-sequences to visualise consensus | * Theo aligned 300 luciferase-sequences to visualise consensus | ||
Revision as of 08:51, 2 August 2010
Monday
Afternoon
- Theo created a plan for an LRE Biobrick, and submitted it to DNA 2.0 for a quote.
- Anja, Hannah and Emily worked on text for Sterilin
- Peter and Will created primer sequences for first experiment
Tuesday
- Duncan provided several E. coli strains. We plated out TOP10 to create colonies from which to create competent cells for transformation.
- We organised all the stock in the lab
- We met with Dr. Summers to discuss quiescence. He was very encouraging but have yet to settle IP concerns. He also suggested considering mopping up Rcd from a leaky inducible promoter with antisense RNA.
Wednesday
Inoculated a broth with Top 10. Making competent cells tomorrow.
Thursday
To do: prepare competent cells
Friday
- Preparing order for sythesis
- Theo aligned 300 luciferase-sequences to visualise consensus
- Peter and Theo discovered DINAmelt, downloaded UNA Fold to model Rcd dynamics
- Anja, Emily and Bill prepared chemically competent cells!
- By spending an hour in a cold room. It was cold but worth it. We got to drink hot chocolate.
